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Dear OpenSPIM users,<br>
I would have a question: In "Stack at this Z plus" it is possible to
set maximum of 1500 microns. Is it possible to circumvent somehow
this limitation? We need to acquire embryonic mouse hearts with
greater diameter, thus having possibility to capture specimens up to
2.5-3 mm in depth would be useful. At the moment I acquire two
consecutive stacks of optical sections for one xy position, but
differing in z. Afterwards I stitch them together. <br>
Moreover, having some tool that would be able to capture
automatically a matrix of overlapping fields of view in xy with the
same depth settings would be superior. I know, it is not easy to
program. I am just describing what would be useful for us, since we
work with relatively big pieces of fixed specimens, up to 4x4x4mm
cubic.<br>
Thank you very much for your help in advance!<br>
Best regards,<br>
Martin<br>
<br>
----------------------<br class="">
<p class="" style="font-family:Calibri,sans-serif; color:#404040;
line-height:120%; text-autospace:none; font-size:11.0pt"> Martin
Capek, Ph.D. <br class="">
Dpt. Biomathematics<br class="">
</p>
<p class="" style="font-family:Calibri,sans-serif; color:#404040;
font-size:10.0pt"> Institute of Physiology ASCR, v.v.i. <br
class="">
Videnska 1083<br class="">
142 20 Praha 4 </p>
<p class="" style="font-family:Calibri,sans-serif; color:#404040;
font-size:10.0pt"> Tel. +420 241 062 334<br class="">
<a moz-do-not-send="true" href="http://fgu.cas.cz/"
target="_blank" class="">http://fgu.cas.cz</a></p>
<br>
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