[OpenSPIM] sCMOS global vs rolling shutter

[Michael Weber]

Dear Aryeh,

rolling shutter gives you twice the speed, but potential artifacts caused by the time offset across the frame. There are cameras which give you both shutter modes (Andor Zyla etc.), if you are willing to give up some QE. It really depends on your specimen, and what kind of analysis you want to do. Unless you want to quantify rapid movements across the frame (e.g. fast fluidics), I would say rolling shutter is fine.

Best,
Michael

On May 26, 2015, at 3:25 PM, Aryeh Weiss <aryeh at CC.HUJI.AC.IL> wrote:

> Should I choose an sCMOS camera with a global shutter (but lower QE)  or one with a rolling  shutter (but higher QE)?
> 
> What is the experience of this group on this question?
> 
> Again, thanks in advance.
> --aryeh
> 
> -- 
> Aryeh Weiss
> Faculty of Engineering
> Bar Ilan University
> Ramat Gan 52900 Israel
> 
> Ph:  972-3-5317638
> FAX: 972-3-7384051
> 
> 
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_____________

Michael Weber
Postdoc, Huisken lab
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108, 01307 Dresden
Tel. 0049 351/2102837

http://www.mpi-cbg.de/huisken

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