<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Dear Aryeh,<div><br></div><div>rolling shutter gives you twice the speed, but potential artifacts caused by the time offset across the frame. There are cameras which give you both shutter modes (Andor Zyla etc.), if you are willing to give up some QE. It really depends on your specimen, and what kind of analysis you want to do. Unless you want to quantify rapid movements across the frame (e.g. fast fluidics), I would say rolling shutter is fine.</div><div><br></div><div>Best,</div><div>Michael</div><div><br><div><div>On May 26, 2015, at 3:25 PM, Aryeh Weiss <<a href="mailto:aryeh@CC.HUJI.AC.IL">aryeh@CC.HUJI.AC.IL</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite">Should I choose an sCMOS camera with a global shutter (but lower QE) or one with a rolling shutter (but higher QE)?<br><br>What is the experience of this group on this question?<br><br>Again, thanks in advance.<br>--aryeh<br><br>-- <br>Aryeh Weiss<br>Faculty of Engineering<br>Bar Ilan University<br>Ramat Gan 52900 Israel<br><br>Ph: 972-3-5317638<br>FAX: 972-3-7384051<br><br><br>_______________________________________________<br>OpenSPIM mailing list<br><a href="mailto:OpenSPIM@openspim.org">OpenSPIM@openspim.org</a><br>http://openspim.org/mailman/listinfo/openspim<br></blockquote></div><br><div apple-content-edited="true">
<span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; border-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; border-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">_____________<br><br>Michael Weber<br>Postdoc, Huisken lab<br>Max Planck Institute of Molecular Cell Biology and Genetics<br>Pfotenhauerstrasse 108, 01307 Dresden<br>Tel. 0049 351/2102837<br><br><a href="http://www.mpi-cbg.de/huisken">http://www.mpi-cbg.de/huisken</a></div></span></div></span></div></span>
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