[OpenSPIM] Alternative to Agarose

[Windell, Dylan]

Hi Arnaud,

I was planning to also try various tubing materials in the future. This paper has a nice comparison of a few different capillary types which you might want to have a look at.

Good luck!

Dylan

Dylan Windell
Postgraduate Researcher
College<http://business-school.exeter.ac.uk/research/areas/centres/isr/> of Life and Environmental Sciences
University of Exeter, Geoffrey Pope
Stocker Road, Exeter EX4 4QD, UK
phone: +44 (0) 7596725056
email: dw388 at exeter.ac.uk<mailto:dw388 at exeter.ac.uk>


From: openspim-bounces at openspim.org [mailto:openspim-bounces at openspim.org] On Behalf Of Eric Edsinger
Sent: 30 June 2015 15:24
To: Arnaud Monnard
Cc: OpenSPIM at openspim.org
Subject: Re: [OpenSPIM] Alternative to Agarose

Hi Arnaud,

I've never used it with OpenSPIM, or on a lightsheet system having a similar design for mounting, but CyGel or CyGel Sustain from BioStatus might work.  It offers improved optical clarity over agarose.  Also, it is a liquid at cold and becomes a solid at room temperature or higher, making it easy to recover live samples.  Its main drawback is cost but otherwise I really liked it when I tried it on a confocal and on a lattice lightsheet with live squid embryos and hatchlings.

I'd be curious to know how it goes, if you or others give it a try - or have tried it.

Good luck!
Eric Edsinger
Postdoctoral researcher
OIST Molecular Genetics Unit



On Tue, Jun 30, 2015 at 11:07 PM, Arnaud Monnard <arnaud.monnard at unibas.ch<mailto:arnaud.monnard at unibas.ch>> wrote:
Dear all,

I wanted to know if anyone would have experience with any alternative to agarose or FEP tube for embedding and imaging samples?
I am currently thinking of using Gelrite, but any suggestion would be more than welcome.

Thanks!

Kind regards

Arnaud Monnard
PhD student
Biozentrum, Basel

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