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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Hi Arnaud,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">I was planning to also try various tubing materials in the future. This paper has a nice comparison of a few different capillary types
which you might want to have a look at.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Good luck!<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Dylan
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Dylan Windell<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Postgraduate Researcher<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><a href="http://business-school.exeter.ac.uk/research/areas/centres/isr/"><span style="color:#1F497D;text-decoration:none">College</span></a> of Life and Environmental
Sciences<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#1F497D">University of Exeter, Geoffrey Pope<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Stocker Road, Exeter EX4 4QD, UK
<o:p></o:p></span></p>
<p class="MsoNormal"><i><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#1F497D">phone: +44 (0) 7596725056<o:p></o:p></span></i></p>
<p class="MsoNormal"><i><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#1F497D">email:
<u><a href="mailto:dw388@exeter.ac.uk">dw388@exeter.ac.uk</a></u><o:p></o:p></span></i></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif"> openspim-bounces@openspim.org [mailto:openspim-bounces@openspim.org]
<b>On Behalf Of </b>Eric Edsinger<br>
<b>Sent:</b> 30 June 2015 15:24<br>
<b>To:</b> Arnaud Monnard<br>
<b>Cc:</b> OpenSPIM@openspim.org<br>
<b>Subject:</b> Re: [OpenSPIM] Alternative to Agarose<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Hi Arnaud,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">I've never used it with OpenSPIM, or on a lightsheet system having a similar design for mounting, but CyGel or CyGel Sustain from BioStatus might work. It offers improved optical clarity over agarose. Also, it is a liquid at cold and
becomes a solid at room temperature or higher, making it easy to recover live samples. Its main drawback is cost but otherwise I really liked it when I tried it on a confocal and on a lattice lightsheet with live squid embryos and hatchlings.<br>
<br>
I'd be curious to know how it goes, if you or others give it a try - or have tried it.<o:p></o:p></p>
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Good luck!<o:p></o:p></p>
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<p class="MsoNormal">Eric Edsinger<o:p></o:p></p>
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<p class="MsoNormal">Postdoctoral researcher<br>
OIST Molecular Genetics Unit<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">On Tue, Jun 30, 2015 at 11:07 PM, Arnaud Monnard <<a href="mailto:arnaud.monnard@unibas.ch" target="_blank">arnaud.monnard@unibas.ch</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US">Dear all,<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US"> <o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US">I wanted to know if anyone would have experience with any alternative to agarose or FEP tube for embedding and imaging samples?<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US">I am currently thinking of using Gelrite, but any suggestion would be more than welcome.<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US"> <o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US">Thanks!<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US"> <o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US">Kind regards<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-US"> <o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="DE-CH">Arnaud Monnard</span><span lang="EN-US"><o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="DE-CH">PhD student</span><span lang="EN-US"><o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="DE-CH">Biozentrum, Basel</span><span lang="EN-US"><o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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