[OpenSPIM] Alternative to Agarose

[Eric Edsinger]

Hi Arnaud,

I've never used it with OpenSPIM, or on a lightsheet system having a
similar design for mounting, but CyGel or CyGel Sustain from BioStatus
might work.  It offers improved optical clarity over agarose.  Also, it is
a liquid at cold and becomes a solid at room temperature or higher, making
it easy to recover live samples.  Its main drawback is cost but otherwise I
really liked it when I tried it on a confocal and on a lattice lightsheet
with live squid embryos and hatchlings.

I'd be curious to know how it goes, if you or others give it a try - or
have tried it.

Good luck!

Eric Edsinger
Postdoctoral researcher
OIST Molecular Genetics Unit



On Tue, Jun 30, 2015 at 11:07 PM, Arnaud Monnard <arnaud.monnard at unibas.ch>
wrote:

>  Dear all,
>
>
>
> I wanted to know if anyone would have experience with any alternative to
> agarose or FEP tube for embedding and imaging samples?
>
> I am currently thinking of using Gelrite, but any suggestion would be more
> than welcome.
>
>
>
> Thanks!
>
>
>
> Kind regards
>
>
>
> Arnaud Monnard
>
> PhD student
>
> Biozentrum, Basel
>
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> OpenSPIM at openspim.org
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>
>
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