[OpenSPIM] Alternative to Agarose

[Arnaud Monnard]

Hi guys,

Thanks for the replies.

I will order the cygel and could let you know how I get on.
I had some inconclusive experiences with matrigel in the past too and would definitely be extremely interested by a gel that would polymerise by another route than temperature change.

Have a nice evening.

Best regards,
Arnaud Monnard.

Le 1 juil. 2015 à 17:06, Eric Edsinger <000generic at gmail.com<mailto:000generic at gmail.com>> a écrit :

Hi Marco,

That's great - I would love to hear about how CyGel works for you for OpenSPIM/lightsheet - and I'm very curious about the two-component hydrogel for live samples that does not require a temperature trigger.  It would be great to hear about it on OpenSPIM if and when you can share details :)

Good luck!
Eric


On Wed, Jul 1, 2015 at 11:08 PM, Marcello, Marco <M.Marcello at liverpool.ac.uk<mailto:M.Marcello at liverpool.ac.uk>> wrote:
Hi,

I am planning to use CyGel soon, I will let you know how it goes. Meanwhile with some colleagues in Chemistry we are working on a two components hydrogel that does not use temperature as gelifying  factor.
It could be useful for live samples that do not tolerate well  variations in temperature. I also used Matrigel in the past.


Best,

Marco

Dr. Marco Marcello
Light Microscopy Facility Manager
Institute of Integrative Biology
University of Liverpool
L69 7ZB
Liverpool
UK
tel +44 (0) 151 7955154<tel:%2B44%20%280%29%20151%207955154>
http://pcwww.liv.ac.uk/~cci/




From: openspim-bounces at openspim.org<mailto:openspim-bounces at openspim.org> [mailto:openspim-bounces at openspim.org<mailto:openspim-bounces at openspim.org>] On Behalf Of Eric Edsinger
Sent: 30 June 2015 15:24
To: Arnaud Monnard
Cc: OpenSPIM at openspim.org<mailto:OpenSPIM at openspim.org>
Subject: Re: [OpenSPIM] Alternative to Agarose

Hi Arnaud,

I've never used it with OpenSPIM, or on a lightsheet system having a similar design for mounting, but CyGel or CyGel Sustain from BioStatus might work.  It offers improved optical clarity over agarose.  Also, it is a liquid at cold and becomes a solid at room temperature or higher, making it easy to recover live samples.  Its main drawback is cost but otherwise I really liked it when I tried it on a confocal and on a lattice lightsheet with live squid embryos and hatchlings.

I'd be curious to know how it goes, if you or others give it a try - or have tried it.

Good luck!
Eric Edsinger
Postdoctoral researcher
OIST Molecular Genetics Unit



On Tue, Jun 30, 2015 at 11:07 PM, Arnaud Monnard <arnaud.monnard at unibas.ch<mailto:arnaud.monnard at unibas.ch>> wrote:
Dear all,

I wanted to know if anyone would have experience with any alternative to agarose or FEP tube for embedding and imaging samples?
I am currently thinking of using Gelrite, but any suggestion would be more than welcome.

Thanks!

Kind regards

Arnaud Monnard
PhD student
Biozentrum, Basel

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