[OpenSPIM] Alternative to Agarose

[Eric Edsinger]

Hi Marco,

That's great - I would love to hear about how CyGel works for you for
OpenSPIM/lightsheet - and I'm very curious about the two-component hydrogel
for live samples that does not require a temperature trigger.  It would be
great to hear about it on OpenSPIM if and when you can share details :)

Good luck!
Eric


On Wed, Jul 1, 2015 at 11:08 PM, Marcello, Marco <M.Marcello at liverpool.ac.uk
> wrote:

>  Hi,
>
>
>
> I am planning to use CyGel soon, I will let you know how it goes.
> Meanwhile with some colleagues in Chemistry we are working on a two
> components hydrogel that does not use temperature as gelifying  factor.
>
> It could be useful for live samples that do not tolerate well  variations
> in temperature. I also used Matrigel in the past.
>
>
>
>
>
> Best,
>
>
>
> Marco
>
>
>
> Dr. Marco Marcello
>
> Light Microscopy Facility Manager
>
> Institute of Integrative Biology
>
> University of Liverpool
>
> L69 7ZB
>
> Liverpool
>
> UK
>
> tel +44 (0) 151 7955154
>
> http://pcwww.liv.ac.uk/~cci/
>
>
>
>
>
>
>
>
>
> *From:* openspim-bounces at openspim.org [mailto:
> openspim-bounces at openspim.org] *On Behalf Of *Eric Edsinger
> *Sent:* 30 June 2015 15:24
> *To:* Arnaud Monnard
> *Cc:* OpenSPIM at openspim.org
> *Subject:* Re: [OpenSPIM] Alternative to Agarose
>
>
>
> Hi Arnaud,
>
>
>
> I've never used it with OpenSPIM, or on a lightsheet system having a
> similar design for mounting, but CyGel or CyGel Sustain from BioStatus
> might work.  It offers improved optical clarity over agarose.  Also, it is
> a liquid at cold and becomes a solid at room temperature or higher, making
> it easy to recover live samples.  Its main drawback is cost but otherwise I
> really liked it when I tried it on a confocal and on a lattice lightsheet
> with live squid embryos and hatchlings.
>
> I'd be curious to know how it goes, if you or others give it a try - or
> have tried it.
>
>
> Good luck!
>
> Eric Edsinger
>
> Postdoctoral researcher
> OIST Molecular Genetics Unit
>
>
>
>
>
>
>
> On Tue, Jun 30, 2015 at 11:07 PM, Arnaud Monnard <arnaud.monnard at unibas.ch>
> wrote:
>
> Dear all,
>
>
>
> I wanted to know if anyone would have experience with any alternative to
> agarose or FEP tube for embedding and imaging samples?
>
> I am currently thinking of using Gelrite, but any suggestion would be more
> than welcome.
>
>
>
> Thanks!
>
>
>
> Kind regards
>
>
>
> Arnaud Monnard
>
> PhD student
>
> Biozentrum, Basel
>
>
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>
>
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