<div dir="ltr">Hi Marco,<div><br></div><div>That's great - I would love to hear about how CyGel works for you for OpenSPIM/lightsheet - and I'm very curious about the two-component hydrogel for live samples that does not require a temperature trigger. It would be great to hear about it on OpenSPIM if and when you can share details :)</div><div><br></div><div>Good luck!</div><div>Eric</div><div> </div></div><div class="gmail_extra"><br><div class="gmail_quote">On Wed, Jul 1, 2015 at 11:08 PM, Marcello, Marco <span dir="ltr"><<a href="mailto:M.Marcello@liverpool.ac.uk" target="_blank">M.Marcello@liverpool.ac.uk</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
<div lang="EN-GB" link="blue" vlink="purple">
<div>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Hi,<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">I am planning to use CyGel soon, I will let you know how it goes. Meanwhile with some colleagues in Chemistry we are working on a two components hydrogel that
does not use temperature as gelifying factor.<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">It could be useful for live samples that do not tolerate well variations in temperature. I also used Matrigel in the past.<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Best,<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Marco<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif""><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Dr. Marco Marcello<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Light Microscopy Facility Manager<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Institute of Integrative Biology<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">University of Liverpool<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">L69 7ZB<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">Liverpool<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">UK
<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d">tel <a href="tel:%2B44%20%280%29%20151%207955154" value="+441517955154" target="_blank">+44 (0) 151 7955154</a><u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><a href="http://pcwww.liv.ac.uk/~cci/" target="_blank">http://pcwww.liv.ac.uk/~cci/</a><u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> <a href="mailto:openspim-bounces@openspim.org" target="_blank">openspim-bounces@openspim.org</a> [mailto:<a href="mailto:openspim-bounces@openspim.org" target="_blank">openspim-bounces@openspim.org</a>]
<b>On Behalf Of </b>Eric Edsinger<br>
<b>Sent:</b> 30 June 2015 15:24<span class=""><br>
<b>To:</b> Arnaud Monnard<br>
<b>Cc:</b> <a href="mailto:OpenSPIM@openspim.org" target="_blank">OpenSPIM@openspim.org</a><br>
<b>Subject:</b> Re: [OpenSPIM] Alternative to Agarose<u></u><u></u></span></span></p>
<p class="MsoNormal"><u></u> <u></u></p>
<div>
<p class="MsoNormal">Hi Arnaud,<u></u><u></u></p><div><div class="h5">
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<p class="MsoNormal">I've never used it with OpenSPIM, or on a lightsheet system having a similar design for mounting, but CyGel or CyGel Sustain from BioStatus might work. It offers improved optical clarity over agarose. Also, it is a liquid at cold and
becomes a solid at room temperature or higher, making it easy to recover live samples. Its main drawback is cost but otherwise I really liked it when I tried it on a confocal and on a lattice lightsheet with live squid embryos and hatchlings.<br>
<br>
I'd be curious to know how it goes, if you or others give it a try - or have tried it.<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal" style="margin-bottom:12.0pt"><br>
Good luck!<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal">Eric Edsinger<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal">Postdoctoral researcher<br>
OIST Molecular Genetics Unit<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
</div></div></div><div><div class="h5">
<div>
<p class="MsoNormal"><u></u> <u></u></p>
<div>
<p class="MsoNormal">On Tue, Jun 30, 2015 at 11:07 PM, Arnaud Monnard <<a href="mailto:arnaud.monnard@unibas.ch" target="_blank">arnaud.monnard@unibas.ch</a>> wrote:<u></u><u></u></p>
<div>
<div>
<p class="MsoNormal"><span lang="EN-US">Dear all,<u></u><u></u></span></p>
<p class="MsoNormal"><span lang="EN-US"> <u></u><u></u></span></p>
<p class="MsoNormal"><span lang="EN-US">I wanted to know if anyone would have experience with any alternative to agarose or FEP tube for embedding and imaging samples?<u></u><u></u></span></p>
<p class="MsoNormal"><span lang="EN-US">I am currently thinking of using Gelrite, but any suggestion would be more than welcome.<u></u><u></u></span></p>
<p class="MsoNormal"><span lang="EN-US"> <u></u><u></u></span></p>
<p class="MsoNormal"><span lang="EN-US">Thanks!<u></u><u></u></span></p>
<p class="MsoNormal"><span lang="EN-US"> <u></u><u></u></span></p>
<p class="MsoNormal"><span lang="EN-US">Kind regards<u></u><u></u></span></p>
<p class="MsoNormal"><span lang="EN-US"> <u></u><u></u></span></p>
<p class="MsoNormal"><span lang="DE-CH">Arnaud Monnard</span><span lang="EN-US"><u></u><u></u></span></p>
<p class="MsoNormal"><span lang="DE-CH">PhD student</span><span lang="EN-US"><u></u><u></u></span></p>
<p class="MsoNormal"><span lang="DE-CH">Biozentrum, Basel</span><span lang="EN-US"><u></u><u></u></span></p>
</div>
</div>
<p class="MsoNormal" style="margin-bottom:12.0pt"><br>
_______________________________________________<br>
OpenSPIM mailing list<br>
<a href="mailto:OpenSPIM@openspim.org" target="_blank">OpenSPIM@openspim.org</a><br>
<a href="http://openspim.org/mailman/listinfo/openspim" target="_blank">http://openspim.org/mailman/listinfo/openspim</a><u></u><u></u></p>
</div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
</div></div></div>
</div>
</blockquote></div><br></div>