[OpenSPIM] Information on T-SPIM construction

[Michael Weber]

Hey David,

Pete might have a step file for the 3-objective chamber. And yes, in principle you just need two arms and a 50/50 splitter, but there are some limitations. Unless you add shutters or AOTFs, you simultaneously illuminate from both sides. Depending on the sample, this might not be ideal, as you get an overlay of "sharp" and "blurry" images. This typically gets worse with larger samples. You can add manual shutters to be able to choose the side of illumination. If you want to be able to quickly record images with sequential double-sided illumination, you need to integrate shutters/AOTFs into the software.

Best,
Michael

On Feb 18, 2015, at 8:41 PM, David Burk <davidburk1972 at gmail.com> wrote:

> I apologize in advance if the answer to my question is somewhere in the archives.
> 
> I am interested in building a T-SPIM system and ran across an archived conversation on this from June 2014. There was mention that such a setup had been built as well as a possible 3D model of the imaging chamber being available. I couldn't find any additional information on the OpenSPIM.org site and just wanted to ask if there was a link to this information somewhere. Based on the 3D model on the site it looks like you really just need to replicate one arm of the laser path and add a 50/50 splitter near the laser. Would there be needed changes to the operating software? If so, that is beyond my (rather limited) capabilities. However, if instructions exist that'd be great!
> 
> I appreciate any information you may have on this.
> 
> Best,
> David
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_____________

Michael Weber
Postdoc, Huisken lab
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108, 01307 Dresden
Tel. 0049 351/2102837

http://www.mpi-cbg.de/huisken

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