<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div>Hey David,</div><div><br></div><div>Pete might have a step file for the 3-objective chamber. And yes, in principle you just need two arms and a 50/50 splitter, but there are some limitations. Unless you add shutters or AOTFs, you simultaneously illuminate from both sides. Depending on the sample, this might not be ideal, as you get an overlay of "sharp" and "blurry" images. This typically gets worse with larger samples. You can add manual shutters to be able to choose the side of illumination. If you want to be able to quickly record images with sequential double-sided illumination, you need to integrate shutters/AOTFs into the software.</div><div><br></div><div>Best,</div><div>Michael</div><br><div><div>On Feb 18, 2015, at 8:41 PM, David Burk <<a href="mailto:davidburk1972@gmail.com">davidburk1972@gmail.com</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div dir="ltr">I apologize in advance if the answer to my question is somewhere in the archives.<div><br></div><div>I am interested in building a T-SPIM system and ran across an archived conversation on this from June 2014. There was mention that such a setup had been built as well as a possible 3D model of the imaging chamber being available. I couldn't find any additional information on the <a href="http://OpenSPIM.org">OpenSPIM.org</a> site and just wanted to ask if there was a link to this information somewhere. Based on the 3D model on the site it looks like you really just need to replicate one arm of the laser path and add a 50/50 splitter near the laser. Would there be needed changes to the operating software? If so, that is beyond my (rather limited) capabilities. However, if instructions exist that'd be great!</div><div><br></div><div>I appreciate any information you may have on this.</div><div><br></div><div>Best,</div><div>David</div></div>
_______________________________________________<br>OpenSPIM mailing list<br><a href="mailto:OpenSPIM@openspim.org">OpenSPIM@openspim.org</a><br>http://openspim.org/mailman/listinfo/openspim<br></blockquote></div><br><div apple-content-edited="true">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; border-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; border-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; border-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">_____________<br><br>Michael Weber<br>Postdoc, Huisken lab<br>Max Planck Institute of Molecular Cell Biology and Genetics<br>Pfotenhauerstrasse 108, 01307 Dresden<br>Tel. 0049 351/2102837<br><br><a href="http://www.mpi-cbg.de/huisken">http://www.mpi-cbg.de/huisken</a></div></span></div></span></div></span>
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