[OpenSPIM] Information on T-SPIM construction

[David Burk]

Thanks, Michael.

Depending on the sample, this might not be ideal, as you get an overlay of
> "sharp" and "blurry" images.

Is this due to the fact that one light sheet, say from the left, may not
have its minimum beam waist at the same position as that from that of the
right sheet? Or more due to scattering, absorption, etc. of the light as it
interacts with the specimen?
I found some images that showed what you are talking about. I just thought
there may be some 'middle ground' you could settle on by adjusting the
sheets from either side that would give relatively uniform illumination
(but with a loss of resolution).
It does sound like - if you choose the slow manual method - this could be
doable. Illuminate from left > capture images > close left shutter >
illuminate from right > capture > merge?
Perhaps detailed designs of a T-SPIM system will eventually be uploaded to
the web along with the modifications to the software to drive the
additional hardware.

Have a great weekend,
David

On Fri, Feb 20, 2015 at 6:40 AM, Michael Weber <weber at mpi-cbg.de> wrote:

> Hey David,
>
> Pete might have a step file for the 3-objective chamber. And yes, in
> principle you just need two arms and a 50/50 splitter, but there are some
> limitations. Unless you add shutters or AOTFs, you simultaneously
> illuminate from both sides. Depending on the sample, this might not be
> ideal, as you get an overlay of "sharp" and "blurry" images. This typically
> gets worse with larger samples. You can add manual shutters to be able to
> choose the side of illumination. If you want to be able to quickly record
> images with sequential double-sided illumination, you need to integrate
> shutters/AOTFs into the software.
>
> Best,
> Michael
>
> On Feb 18, 2015, at 8:41 PM, David Burk <davidburk1972 at gmail.com> wrote:
>
> I apologize in advance if the answer to my question is somewhere in the
> archives.
>
> I am interested in building a T-SPIM system and ran across an archived
> conversation on this from June 2014. There was mention that such a setup
> had been built as well as a possible 3D model of the imaging chamber being
> available. I couldn't find any additional information on the OpenSPIM.org
> site and just wanted to ask if there was a link to this information
> somewhere. Based on the 3D model on the site it looks like you really just
> need to replicate one arm of the laser path and add a 50/50 splitter near
> the laser. Would there be needed changes to the operating software? If so,
> that is beyond my (rather limited) capabilities. However, if instructions
> exist that'd be great!
>
> I appreciate any information you may have on this.
>
> Best,
> David
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>
> _____________
>
> Michael Weber
> Postdoc, Huisken lab
> Max Planck Institute of Molecular Cell Biology and Genetics
> Pfotenhauerstrasse 108, 01307 Dresden
> Tel. 0049 351/2102837
>
> http://www.mpi-cbg.de/huisken
>
>
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