[OpenSPIM] obis laser

Neil Anthony nantho2 at emory.edu
Fri Jun 27 09:48:12 CDT 2014


Hi Guys,

might I ask what the issue is with the OBIS that requires a clean up?  I 
guess I'm wrong in thinking that a diode laser would only emit one 
wavelength (or very small spectral bandwidth)?

Thanks
Neil

On 6/12/2014 3:58 AM, pitrone wrote:
> Hello Michael,
>
> We did use OBIS for a few months due to a defective CUBE, and it 
> worked very well... But at the time we did not have it integrated into 
> the plugin, so unless the driver can be merged into it then I cannot 
> make any statement for or against getting one. Otherwise when this 
> merger is accomplished I would say go for it, with the caveat of 
> getting a clean up filter (eGFP excitation of 490/10 or 490/15 ±400 
> USD/GBP/EUR) along side it.
>
> My $0.02,
> Pete
>
> Peter Gabriel Pitrone FRMS TechRMS
> Microscopist & Imaging Specialist
> Dr. Pavel Tomancak's group
> Max Planck Institute for Molecular
> Cell Biology and Genetics (MPI-CBG)
> Pfotenhauerstrasse 108
> 01307, Dresden
> Germany
>
> +49 (0) 351 210 2687
> pitrone at mpi-cbg.de <mailto:pitrone at mpi-cbg.de>
> http://www.openspim.org
>
>
>
> On Jun 11, 2014, at 7:37 PM, Kevin W Eliceiri wrote:
>
>> Hi Michael,
>>
>> I know our colleagues in Germany have used both and been pleased. 
>> Only issue was need cleanup filter in both cases. There are now 
>> drivers on github for micromanger control of obis
>> but we have not to my knowledge tried this. See 
>> https://github.com/hc5/CoherentOBIS
>>
>>
>> best
>> kevin
>>
>> On 06/11/14, Michael Redd  wrote:
>>>
>>>
>>>
>>> Coherent is replacing their Cube laser with their Obis line. The 
>>> Obis is USB connectable and is much cheaper than the Cube. Has 
>>> anyone used an Obis laser with OpenSPIM or micromanager?
>>>
>>>
>>> Cheers
>>> mike
>>
>> --
>> Kevin W. Eliceiri
>> Director, Laboratory for Optical and Computational Instrumentation (LOCI)
>> Departments Cell and Molecular Biology and Biomedical Engineering
>> Affiliate Principal Investigator, Morgridge Institute for Research (MIR)
>> Room 271 Animal Sciences, 1675 Observatory Drive, Madison, WI 53706
>> Phone: 608-263-6288
>>
>> _______________________________________________
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>

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