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Hi Guys,<br>
<br>
might I ask what the issue is with the OBIS that requires a clean
up? I guess I'm wrong in thinking that a diode laser would only
emit one wavelength (or very small spectral bandwidth)?<br>
<br>
Thanks<br>
Neil<br>
<br>
<div class="moz-cite-prefix">On 6/12/2014 3:58 AM, pitrone wrote:<br>
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cite="mid:79326BE1-0617-4958-95A3-E66937948BC0@mpi-cbg.de"
type="cite">
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<div>Hello Michael,</div>
<div><br>
</div>
<div>We did use OBIS for a few months due to a defective CUBE, and
it worked very well… But at the time we did not have it
integrated into the plugin, so unless the driver can be merged
into it then I cannot make any statement for or against getting
one. Otherwise when this merger is accomplished I would say go
for it, with the caveat of getting a clean up filter (eGFP
excitation of 490/10 or 490/15 ±400 USD/GBP/EUR) along side it.</div>
<div><br>
</div>
<div>My $0.02,</div>
<div>Pete</div>
<br>
<div apple-content-edited="true">
<span class="Apple-style-span" style="border-collapse: separate;
color: rgb(0, 0, 0); font-family: Helvetica; font-style:
normal; font-variant: normal; font-weight: normal;
letter-spacing: normal; line-height: normal; orphans: 2;
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none; white-space: normal; widows: 2; word-spacing: 0px;
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-webkit-border-vertical-spacing: 0px;
-webkit-text-decorations-in-effect: none;
-webkit-text-size-adjust: auto; -webkit-text-stroke-width:
0px; font-size: medium; ">Peter Gabriel Pitrone FRMS TechRMS<br>
Microscopist & Imaging Specialist<br>
Dr. Pavel Tomancak's group<br>
Max Planck Institute for Molecular<br>
Cell Biology and Genetics (MPI-CBG)<br>
Pfotenhauerstrasse 108<br>
01307, Dresden<br>
Germany<br>
<br>
+49 (0) 351 210 2687<br>
<a moz-do-not-send="true" href="mailto:pitrone@mpi-cbg.de">pitrone@mpi-cbg.de</a><br>
<a class="moz-txt-link-freetext" href="http://www.openspim.org">http://www.openspim.org</a><br>
<br>
<br>
</span>
</div>
<br>
<div>
<div>On Jun 11, 2014, at 7:37 PM, Kevin W Eliceiri wrote:</div>
<br class="Apple-interchange-newline">
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<div>Hi Michael,<br>
<br>
I know our colleagues in Germany have used both and been
pleased. Only issue was need cleanup filter in both cases.
There are now drivers on github for micromanger control of
obis<br>
but we have not to my knowledge tried this. See <a
moz-do-not-send="true"
href="https://github.com/hc5/CoherentOBIS">https://github.com/hc5/CoherentOBIS</a><br>
<br>
<br>
best<br>
kevin<br>
<br>
On 06/11/14, Michael Redd wrote:<br>
<blockquote type="cite"><br>
</blockquote>
<blockquote type="cite"><br>
</blockquote>
<blockquote type="cite"><br>
</blockquote>
<blockquote type="cite">Coherent is replacing their Cube
laser with their Obis line. The Obis is USB connectable
and is much cheaper than the Cube. Has anyone used an Obis
laser with OpenSPIM or micromanager?<br>
</blockquote>
<blockquote type="cite"><br>
</blockquote>
<blockquote type="cite"><br>
</blockquote>
<blockquote type="cite">Cheers<br>
</blockquote>
<blockquote type="cite">mike<br>
</blockquote>
<br>
--<br>
Kevin W. Eliceiri<br>
Director, Laboratory for Optical and Computational
Instrumentation (LOCI)<br>
Departments Cell and Molecular Biology and Biomedical
Engineering<br>
Affiliate Principal Investigator, Morgridge Institute for
Research (MIR)<br>
Room 271 Animal Sciences, 1675 Observatory Drive, Madison,
WI 53706<br>
Phone: 608-263-6288<br>
<br>
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