[OpenSPIM] Picard stage error after update

[Veli-Pekka Ronkainen]

Dear all,


We've been scanning some mouse tissue test samples with our dual-side illumination OpenSPIM for a short while now, and we are very happy with the first results!


We have used the original software package downloaded from openspim.org without any updates, and it has been working fine. However, now after using the Fiji updater Picard USB-stage rotation angle coordinates are not right anymore. Changing angle from -180 deg to 180 deg rotates the sample holder only ~180 degrees, and rotation in general spins the sample much less than indicated by the slider. In addition, we can not access and modify old .cfg-files, so that it would be possible to change step size of the twister from 1.8 to 1, as it was discussed earlier: http://openspim.org/pipermail/openspim/2014-July/000482.html . Modification of an existing configuration starts with an error "Error in device Picard Z Stage: Unable to communicate with the device" followed by lots of COM and "No device with label _device name_" -errors. Creating a new configuration doesn't help since it is not possible to add Picard stage z-axis to the config file or modify the serial number.


We've tried to copy the latest Picard .dll-files from the recent Micro-Manager nightly build but the problem persists. Are there some other files we should copy and replace as well? Of course we can still download and configure the non-updated package from openspim.org and be happy using that older version without any problems. Still it would be nice to be up-to-date. Anyone having the same problems or some solutions? Thanks!


Regards,

Veli-Pekka Ronkainen

Light Microscopy Core Facility

Biocenter Oulu, University of Oulu

Finland

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