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<p>Dear all,</p>
<p><br>
</p>
<p>We've been scanning some mouse tissue test samples with our dual-side illumination OpenSPIM for a short while now, and we are very happy with the first results!
<br>
</p>
<p><br>
</p>
<p>We have used the original software package downloaded from openspim.org without any updates, and it has been working fine. However, now after using the Fiji updater Picard USB-stage rotation angle coordinates are not right anymore. Changing angle from -180
deg to 180 deg rotates the sample holder only ~180 degrees, and rotation in general spins the sample much less than indicated by the slider. In addition, we can not access and modify old .cfg-files, so that it would be possible to change step size of the twister
from 1.8 to 1, as it was discussed earlier: <a href="http://openspim.org/pipermail/openspim/2014-July/000482.html">
http://openspim.org/pipermail/openspim/2014-July/000482.html</a> . Modification of an existing configuration starts with an error "Error in device Picard Z Stage: Unable to communicate with the device" followed by lots of COM and "No device with label _device
name_" -errors. Creating a new configuration doesn't help since it is not possible to add Picard stage z-axis to the config file or modify the serial number.
</p>
<p><br>
</p>
<p>We've tried to copy the latest Picard .dll-files from the recent Micro-Manager nightly build but the problem persists. Are there some other files we should copy and replace as well? Of course we can still download and configure the non-updated package from
openspim.org and be happy using that older version without any problems. Still it would be nice to be up-to-date. Anyone having the same problems or some solutions? Thanks!</p>
<p><br>
</p>
<p>Regards,</p>
<p>Veli-Pekka Ronkainen</p>
<p>Light Microscopy Core Facility </p>
<p>Biocenter Oulu, University of Oulu</p>
<p>Finland<br>
</p>
<p><br>
</p>
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