[OpenSPIM] X-SPIM

[Jan Huisken]

Hi Rüdiger,

why do you think you need a 4-lens SPIM?

For most purposes a 3-lens SPIM (or even a 2-lens SPIM) is just fine. The 4-lens system requires a second detection arm, including another camera, which is one of the most expensive components of the setup. The main advantage of the 4-lens system is the ability to record the back side of the sample right after the front side without rotation. When the two cameras are well aligned, both views are automatically registered. This pays off when you want to process the data in realtime [Schmid, 2013; Krzic, 2012]. Otherwise, there is not much of a speed advantage comparing it with a three-lens SPIM with a fast rotational motor.

Here are some examples of 4-lens SPIM setups.

Schmid, B. et al. High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics. Nat. Commun. 4, 2207 (2013). 

Weber, M. & Huisken, J. Omnidirectional microscopy. Nat. Methods 9, 656–657 (2012). 

Tomer, R., Khairy, K., Amat, F. & Keller, P. J. Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy. Nat. Methods 9, 755–763 (2012). 

Krzic, U., Gunther, S., Saunders, T. E., Streichan, S. J. & Hufnagel, L. Multiview light-sheet microscope for rapid in toto imaging. Nat. Methods 9, 730–733 (2012). 

Best
Jan

Dr. Jan Huisken
Center for Systems Biology Dresden
MPI of Molecular Cell Biology and Genetics
Pfotenhauerstr. 108, 01307 Dresden, Germany

On 08 Apr 2014, at 14:36, Rüdiger Rudolf <r.rudolf at hs-mannheim.de> wrote:

> Dear OPEN-SPIM team,
> 
> your website is terrific!
> Since a while we are now getting more and more interested in setting up our own SPIM for a multitude of potential applications.
> My impression is, that for many purposes a dual color-X-SPIM would be needed.
> You show nice pictures on your page of such a system (X-SPIM), but in the parts list the corresponding chamber is not listed.
> Do you, in general, sell the parts listed under „OPEN SPIM“ at the prices indicated on your page and do you also distribute the chambers for X-SPIM?
> 
> Thanks a lot in advance, looking forward to hearing from you!
> Best regards, Rüdiger
> 
> ******************************************************
> Dr. Rüdiger Rudolf, Professor of Biosensorics
> 
> University of Applied Sciences Mannheim
> Faculty of Biotechnology
> Institute of Molecular and Cell Biology
> Paul-Wittsack-Strasse 10
> 68163 Mannheim
> Tel: +49 (0)621 2926804
> Email: r.rudolf at hs-mannheim.de
> ******************************************************
> 
> 
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