[OpenSPIM] Picard Stage Adapter problem (Luke Stuyvenberg) and light sheet thickness

Aurelia Honerkamp-Smith aureliaomega at gmail.com
Fri Sep 27 06:03:31 CDT 2013


Perfect, the 26 September nightly build version of the PVCAM adapter has
cleared up my live view problem!  Thanks very much, I am so happy!
Aurelia



On Fri, Sep 27, 2013 at 4:15 AM, Luke Stuyvenberg <stuyvenberg at wisc.edu>wrote:

> Hi Aurelia,
>
>
> On Thu, 26 Sep 2013, Aurelia Honerkamp-Smith wrote:
>
>  Thanks for this latest update: it has almost fixed my PVCAM problem.
>> Now I can load the camera, and since the last update I no longer get a
>> time-out error message when I use the live view.  But, the live window
>> opens and displays an image, then it doesn't refresh, so I see the same
>> image until I stop and start live view again.  I can now run the Open
>> SPIM plugin, but have to keep stopping and starting live view in order
>> to see what is going on with my calibration sample.  I'm so close!  Any
>> suggestions for fixing this?  Live view through my camera works fine in
>> a plain, non-Fiji and non-OpenSPIM installation of Micromanager, so I
>> can align and take pictures at the moment, but I want to use your lovely
>> plugin.
>>
> Yes, I seem to remember other reports of this trouble (also with PVCAM, if
> I recall correctly), though I had thought them resolved... I believe
> Johannes is correct, and synchronizing with Micro-Manager may resolve this
> issue. I've started work on this, but the process is (frustratingly)
> intricate, and might take some time. In the future I hope to make the
> process unnecessary.
>
> As a temporary measure, you should have no trouble using the PVCAM device
> adapter from the Micro-Manager nightly build (The file should be wherever
> you installed the 'vanilla' Micro-Manager to; simply copy it to OpenSPIM's
> "mm/win32" directory -- it may ask you to overwrite). If you decide to try
> this, please let me know how it works out.
>
> Thanks,
> Luke
>
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