[OpenSPIM] Picard Stage Adapter problem (Luke Stuyvenberg) and light sheet thickness

[Luke Stuyvenberg]

Hi Aurelia,

On Thu, 26 Sep 2013, Aurelia Honerkamp-Smith wrote:

> Thanks for this latest update: it has almost fixed my PVCAM problem.
> Now I can load the camera, and since the last update I no longer get a
> time-out error message when I use the live view.  But, the live window
> opens and displays an image, then it doesn't refresh, so I see the same
> image until I stop and start live view again.  I can now run the Open
> SPIM plugin, but have to keep stopping and starting live view in order
> to see what is going on with my calibration sample.  I'm so close!  Any
> suggestions for fixing this?  Live view through my camera works fine in
> a plain, non-Fiji and non-OpenSPIM installation of Micromanager, so I
> can align and take pictures at the moment, but I want to use your lovely
> plugin.
Yes, I seem to remember other reports of this trouble (also with PVCAM, 
if I recall correctly), though I had thought them resolved... I believe 
Johannes is correct, and synchronizing with Micro-Manager may resolve 
this issue. I've started work on this, but the process is 
(frustratingly) intricate, and might take some time. In the future I 
hope to make the process unnecessary.

As a temporary measure, you should have no trouble using the PVCAM 
device adapter from the Micro-Manager nightly build (The file should be 
wherever you installed the 'vanilla' Micro-Manager to; simply copy it to 
OpenSPIM's "mm/win32" directory -- it may ask you to overwrite). If you 
decide to try this, please let me know how it works out.

Thanks,
Luke