[OpenSPIM] Temperature control for the SPIM chamber

[Michael Weber]

Hi Edgar,

I found the perfusion system more flexible and easier to handle. You can quickly exchange medium (as you mentioned) but also use it just for heating.

Regarding the Aluminum, it's the same reason why people avoid putting Aluminum wheels on their cars during winter time, when salt is used to melt down the ice. The salt in combination with water will form a nasty layer of something on the Aluminum, don't ask me for the details. We saw the very same effect in a chamber made of Aluminum that was filled with PBS.

Best,
Michael

On Sep 24, 2013, at 11:59 AM, edgar.escobar.nieto at ipt.fraunhofer.de wrote:

> Dear Michael,
> 
> Thanks for your recommendation about the oxidation of the Aluminum, I was searching on Internet about that
> but I didn't find anything concrete. I have to ask first in the workshop here in the Institute if it is possible
> to machine Stainless Steel. 
> Could I ask why you use a perfusion system in the SPIM chamber? Is it for changing the media constantly?
> 
> Kind regards,
> Edgar
> _________________________________________________________________________
> 
> Fraunhofer-Institut für Produktionstechnologie IPT 
> Edgar Escobar Nieto 
> 
> 
> 
> 
> Steinbachstraße 17 
> 52074 Aachen 
> 
> edgar.escobar.nieto at ipt.fraunhofer.de 
> http://www.ipt.fraunhofer.de 
> _________________________________________________________________________ 
> 
> 
> 

_____________

Michael Weber
PhD Student, Huisken lab
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108, 01307 Dresden
Tel. 0049 351/2102837

http://www.mpi-cbg.de/huisken

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