[OpenSPIM] About the SPIMage processing tutorial

edgar.escobar.nieto at ipt.fraunhofer.de edgar.escobar.nieto at ipt.fraunhofer.de
Fri Jul 26 03:34:42 CDT 2013


 Hi Johannes,

Thanks a lot. Now I am able to perform the Fusion successfully.

Kind regards,
Edgar Escobar Nieto
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Edgar Escobar Nieto  
 

 
 
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-----Johannes Schindelin <Johannes.Schindelin at gmx.de> schrieb: -----
An: edgar.escobar.nieto at ipt.fraunhofer.de
Von: Johannes Schindelin <Johannes.Schindelin at gmx.de>
Datum: 25.07.2013 19:21
Kopie: openspim at openspim.org
Betreff: Re: About the SPIMage processing tutorial

Hi Edgar,

On Thu, 25 Jul 2013, edgar.escobar.nieto at ipt.fraunhofer.de wrote:

> I am having some issues about the RAM memory when I am trying to perform
> the Fusion of registered multiview OpenSPIM data into a single output
> image using content based fusion.
> 
> I could perform the first step of the tutorial where the output image is
> downsampled 4 times, but when trying to perform the no downsampled but
> cropped output image it was not possible because of lack of RAM memory.

We also experienced those problems when working with a 32-bit Fiji. To
work around the limitations of 32-bit (where effectively only a fraction
of your RAM is accessible due to only 31 bit being available to address
it), we also installed a 64-bit Fiji from http://fiji.sc/ and ran the
fusion there. Maybe that works for you, too?

Ciao,
Johannes
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