[OpenSPIM] About the SPIMage processing tutorial

Johannes Schindelin Johannes.Schindelin at gmx.de
Thu Jul 25 12:21:07 CDT 2013


Hi Edgar,

On Thu, 25 Jul 2013, edgar.escobar.nieto at ipt.fraunhofer.de wrote:

> I am having some issues about the RAM memory when I am trying to perform
> the Fusion of registered multiview OpenSPIM data into a single output
> image using content based fusion.
> 
> I could perform the first step of the tutorial where the output image is
> downsampled 4 times, but when trying to perform the no downsampled but
> cropped output image it was not possible because of lack of RAM memory.

We also experienced those problems when working with a 32-bit Fiji. To
work around the limitations of 32-bit (where effectively only a fraction
of your RAM is accessible due to only 31 bit being available to address
it), we also installed a 64-bit Fiji from http://fiji.sc/ and ran the
fusion there. Maybe that works for you, too?

Ciao,
Johannes




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