[OpenSPIM] Calibration using the target grid and mirror?

[Emmanuel Reynaud]

Kathleen,

Well, it depends on your micrometer slide... if it is an engraved coverslip
like the eppendorf system then you can glue it on a capillary that fits the
tip of the syringe (uncut) and mount it like that.
Basically, you take a syringe, insert a capillary in the tip, glue it, then
glue the grid/mirror on top of the capillary
Stability of the system is very variable. some system well isolated with no
moving parts and other motor vibrating around can be calibrated once before
the set of experiments and of you go and just check at the end for
security. Just a bit of try and error will tell you the best.



*Dr Emmanuel G. Reynaud*
Principal Investigator, Integrative Biology
Conway Institute of Biomolecular and Biomedical Science
UCD Centre for Biomedical Engineering (
http://www.ucd.ie/biomedicalengineering/)

e-mail: emmanuel.reynaud at ucd.ie <matthias.wilm at ucd.ie>
Website:http://cellbiology.ucd.ie/

On 12 October 2017 at 17:18, Kathleen Chen <chens18 at rpi.edu> wrote:

> Hello everyone,
>
> From the wiki there is a method for calibration of the light sheet using a
> grid (from a micrometer slide) and mirror attached to a syringe. We have
> been having some trouble with calibration  in our prototype OpenSPIM system
> and we have a few questions.
>
> 1. How did others extract the small grid from the calibration slide? How
> was it attached to the calibration sample holder (syringe)?
> 2. Is the system calibrated every time before use or is it calibrated less
> frequently? If it is calibrated often, how do you ensure that the grid is
> stable for each position and it doesn't move ("wobble around") in the
> chamber during the calibration?
>
> Would anyone have any advice about this?
>
> Thank you,
> Kathleen
>
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