[OpenSPIM] fluorophores for alignment

[Weber, Michael]

I all you want to see is the laser beam in the field of view than you’re good with water and no emission filter.

Best,
Michael

> On Jun 15, 2017, at 11:19 AM, Feinstein, Timothy N <tnf8 at pitt.edu> wrote:
> 
> We can see the laser well enough using about 1% phenol red in water, at least with 488 and 561 nm lasers.  I have not tried 647 nm.  This is for the alignment technique where you remove the cylinder lens correct?
> 
> Best, 
> 
> 
> Tim
> 
> Timothy Feinstein, Ph.D. 
> Research Scientist
> University of Pittsburgh Department of Developmental Biology
> 
> 
> On 6/15/17, 10:30 AM, "OpenSPIM on behalf of Dr S. Hoehn" <openspim-bounces at openspim.org on behalf of S.Hoehn at damtp.cam.ac.uk> wrote:
> 
>    Hello,
> 
>    Can anyone recommend (cheap) fluorophores that are fluorescent in water 
>    for alignment purposes?
> 
>    We'd specifically need one for a 637nm laser, but fluorophores for the 
>    488nm and 534nm laser line would also be helpful.
> 
>    Thanks a lot.
> 
>    Best wishes,
>    Stephanie
> 
> 
>    Dr. Stephanie Hoehn
>    DAMTP, Biological Physics
>    Centre for Mathematical Sciences
>    Wilberforce Road
>    Cambridge
>    CB3 0WA
>    United Kingdom
>    Office Tel: 01223 337859
>    Office: H0.03
> 
> 
> 
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