[OpenSPIM] fluorophores for alignment

[Feinstein, Timothy N]

We can see the laser well enough using about 1% phenol red in water, at least with 488 and 561 nm lasers.  I have not tried 647 nm.  This is for the alignment technique where you remove the cylinder lens correct?

Best, 


Tim

Timothy Feinstein, Ph.D. 
Research Scientist
University of Pittsburgh Department of Developmental Biology


On 6/15/17, 10:30 AM, "OpenSPIM on behalf of Dr S. Hoehn" <openspim-bounces at openspim.org on behalf of S.Hoehn at damtp.cam.ac.uk> wrote:

    Hello,
    
    Can anyone recommend (cheap) fluorophores that are fluorescent in water 
    for alignment purposes?
    
    We'd specifically need one for a 637nm laser, but fluorophores for the 
    488nm and 534nm laser line would also be helpful.
    
    Thanks a lot.
    
    Best wishes,
    Stephanie
    
    
    Dr. Stephanie Hoehn
    DAMTP, Biological Physics
    Centre for Mathematical Sciences
    Wilberforce Road
    Cambridge
    CB3 0WA
    United Kingdom
    Office Tel: 01223 337859
    Office: H0.03
    
    
    
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