[OpenSPIM] Zebrafish embryo sample preparation

[Nikita Vladimirov]

Hi, Nichelle,
We used a custom-made fish holder (see attached paper, suppl.,Fig.1) to
hold 5-7 dpf zebrafish in SPIM, while it's head and tail are free in water.
The holder surrounds the central segment of fish body (like a slit), and
holds him at place with a thin layer of agarose. Fish was paralyzed or
sedated with MS222, otherwise it would swim out. The agarose is used only
between the mid-body and the holder, and the rest of fish is surrounded by
water. Making the holder and mounting the fish are a bit tricky, but I am
happy to share details if anyone is interested.
[image: Inline image 1]
Best,
Nikita Vladimirov



On Sat, Jun 10, 2017 at 6:49 PM, Nichelle Jackson <nijack at umich.edu> wrote:

> Hello,
>
> Currently we are trying to find the best way to hold a prepared sample in
> FEP tubing in the sample pulley so we can rotate it without shaking. We
> have tried attaching the FEP tube to a syringe which sits on top of the
> sample pulley and drilling a small whole through a rubber stopper, however
> upon rotating the sample, there was a pretty dramatic shift. I was
> wondering if there are deigns for the sample holder shown on the OpenSPIM
> Wiki, or other suggestions for holding a sample prepared for longterm
> imaging of live zebrafish.
>
> Best,
> Nichelle Jackson
>
> Ph.D. Student
> Department of Pharmacology
> University of Michigan, Ann Arbor, MI
> nijack at umich.edu
>
> _______________________________________________
> OpenSPIM mailing list
> OpenSPIM at openspim.org
> http://openspim.org/mailman/listinfo/openspim
>
>
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