[OpenSPIM] Suboptimal acquisition speed

[Peter Gabriel Pitrone]

Hello Tim,

Two questions need answering first:

1.) Are you in "Lightsheet mode"?!

This would "half" your frame rate, as the camera would read in only one direction (top to bottom) instead of bi-directional (from the center to the top and bottom).

And

2.) Assuming you are using the Picard stage: What is your motor dwell time set to?

As a standard setting it is set to 10 ms as to settle the sample from swinging, which could be your issue if you already set your exposure to 10 ms...

I hope that helps explain the problems you could be facing, if not good luck finding what else it could be.


Regards,
Pete

----- Original Message -----
From: "Feinstein, Timothy N" <tnf8 at pitt.edu>
To: openspim at openspim.org
Sent: Monday, March 21, 2016 7:34:02 PM
Subject: [OpenSPIM] Suboptimal acquisition speed

Hello all, 



Does anyone have experience troubleshooting frame rate problems? We have a Hamamatsu Flash 4.0 v2 that is theoretically capable of 100 FPS with a CameraLink card, but we get about half that using Micro-Manager/openSPIM. I have spent some time with Hamamatsu engineers and as far as we can tell the hardware works fine; we can reach expected speeds with Hamamatsu’s diagnostic software (XCAP). Rather it seems like MM is inefficiently communicating with the API, possibly not ‘expecting’ or ‘asking for’ frames at the same interval that they are being delivered from the camera. I really do not want to pay huge premium for Metamorph unless we absolutely have to, especially without knowing whether that will help. 



Thanks for any suggestions. 


Best, 




Tim 



Timothy Feinstein, Ph.D. 

Research Scientist 

University of Pittsburgh Department of Developmental Biology 



_______________________________________________
OpenSPIM mailing list
OpenSPIM at openspim.org
http://openspim.org/mailman/listinfo/openspim