[OpenSPIM] Suboptimal acquisition speed

[Feinstein, Timothy N]

Hello all,

Does anyone have experience troubleshooting frame rate problems?  We have a Hamamatsu Flash 4.0 v2 that is theoretically capable of 100 FPS with a CameraLink card, but we get about half that using Micro-Manager/openSPIM.  I have spent some time with Hamamatsu engineers and as far as we can tell the hardware works fine; we can reach expected speeds with Hamamatsu's diagnostic software (XCAP).  Rather it seems like MM is inefficiently communicating with the API, possibly not 'expecting' or 'asking for' frames at the same interval that they are being delivered from the camera.  I really do not want to pay huge premium for Metamorph unless we absolutely have to, especially without knowing whether that will help.

Thanks for any suggestions.

Best,


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology

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