[OpenSPIM] The shape of light-sheet from a illumination objective

[Monika Pawłowska]

Sounds like your distances are off. Check out the tips in the 'Assembling  
the telescope' instruction  
http://openspim.org/Install_illumination_axis_on_the_optical_breadboard_-_Part_2#Assembling_the_telescope

Best,
Monika

W dniu .12.2016 o 04:24 胡衍 <huyan1020 at sjtu.edu.cn> pisze:

> Dear openSPIM list,
>
> This is Yan, from China.
> I built a light-sheet microscopy following the openSPIM,
> but I found the light sheet shape changing from vertical to horizontal.
>
> The optic path followed the openSPIM,
> There was a vertical light sheet in front of the illumination objective.
> This sheet focused in the position of working distance away from  
> illumination objective,
> the thinnest sheet in vertical direction.
> But after this position the vertical direction height became smaller,
> and the horizontal direction width became larger.
> There is a position long away from the illumination position,
> the light sheet became thinnest in the horizontal direction.
>
> Is there anyone knowing such thing? What is the problem?
> I don't know the reason, so I don't know how to change the optic path.
>
> I am looking forward for your reply.
> Best regards,
> Yan
>
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-- 
Dr Monika Pawłowska
Nencki Institute
02-093 Warsaw
Pasteura 3
Poland