[OpenSPIM] mutli-channel OpenSPIM

[Feinstein, Timothy N]

Hi Kurt,

I pasted below a response to a similar question posed earlier to the list.  I am quite happy with a four-laser launch made by Vortran in California (no commercial interest).  The only downside is that OpenSpim software cannot currently control multi-laser launches.  I currently make do with Micro-Manager, a 3D SpaceNavigator mouse for coarse sample movement and the software that comes with the Picard motorized stage for fine movement.

Best,


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology

---------

Hi Matt,

We use a 488/561 Versalase with an ESio TTL controller to enhance
switching speed.  Our setup largely followed a design that was generously
provided by Johannes Girstmair in the Telford lab at University College
London, with a few modifications to fit our needs.  He provided me
extensive help that I can pass along if he has not done already.

Overall it works quite well.  If you use a TTL controller, you will want
to add the following to the Configuration Settings window in
Micro-Manager:

Laser_[A/B/C/D]_LaserEmission
Laser_[color]_DigitalPeakPowerSetting
TTL_State (I rename this setting 'color' and name the TTL states that
result in the desired outputs 'green', 'red' and 'off'.  This lets me set
multi-channel images by assigning the 'color' variable in Multi-D
Acquisition)

Turn on DigitalModulation in the Device Property Browser and you should be
set.  You have to switch LaserEmission on each time you boot up MM and if
you use a TTL controller PeakPower will determine the laser intensity.

Blanking mode will theoretically help acquire faster with minimized light
exposure.  However I have not got that to work; Micro-Manager's
auto-shutter seems to do the job well enough.

All the best,


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology
From: OpenSPIM <openspim-bounces at openspim.org<mailto:openspim-bounces at openspim.org>> on behalf of "Weiss, Kurt" <KWeiss at morgridge.org<mailto:KWeiss at morgridge.org>>
Date: Monday, December 5, 2016 at 5:22 PM
To: "openspim at openspim.org<mailto:openspim at openspim.org>" <openspim at openspim.org<mailto:openspim at openspim.org>>
Subject: [OpenSPIM] mutli-channel OpenSPIM

Hi,

We are interested in adding more laser channels to our current OpenSPIM. There seem to be three options: (1) multi-channel lauch, (2) laser combiner unit, (3) combine with mirror + dichroic.

If we go for option 1: Does anyone have experience with mult-channel modules which integrate well with the OpenSPIM platform? (Besides the Stradus Versalase I have seen mentioned on this forum, though updates on this unit might be helpful if anyone has strong feelings).

Any other comments on options 2 or 3 would also be appreciated. A few concerns have been addressed here:
http://openspim.org/pipermail/openspim/2013-December/001268.html

Thanks,
Kurt
_______________________
Kurt Weiss, Ph.D.
Postdoctoral Associate
Huisken Group, Medical Engineering
Morgridge Institute for Research
330 N Charter St.
Madison, WI 53715
KWeiss at morgridge.org<mailto:KWeiss at morgridge.org>
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