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<div>Hi Kurt, </div>
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<div>I pasted below a response to a similar question posed earlier to the list. I am quite happy with a four-laser launch made by Vortran in California (no commercial interest). The only downside is that OpenSpim software cannot currently control multi-laser
launches. I currently make do with Micro-Manager, a 3D SpaceNavigator mouse for coarse sample movement and the software that comes with the Picard motorized stage for fine movement. </div>
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<div>Best, </div>
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<div>Tim</div>
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<div>Timothy Feinstein, Ph.D. </div>
<div>Research Scientist</div>
<div>University of Pittsburgh Department of Developmental Biology</div>
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<div style="font-family: Consolas;">Hi Matt, </div>
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<div style="font-family: Consolas;">We use a 488/561 Versalase with an ESio TTL controller to enhance</div>
<div style="font-family: Consolas;">switching speed. Our setup largely followed a design that was generously</div>
<div style="font-family: Consolas;">provided by Johannes Girstmair in the Telford lab at University College</div>
<div style="font-family: Consolas;">London, with a few modifications to fit our needs. He provided me</div>
<div style="font-family: Consolas;">extensive help that I can pass along if he has not done already.</div>
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<div style="font-family: Consolas;">Overall it works quite well. If you use a TTL controller, you will want</div>
<div style="font-family: Consolas;">to add the following to the Configuration Settings window in</div>
<div style="font-family: Consolas;">Micro-Manager: </div>
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<div style="font-family: Consolas;">Laser_[A/B/C/D]_LaserEmission</div>
<div style="font-family: Consolas;">Laser_[color]_DigitalPeakPowerSetting</div>
<div style="font-family: Consolas;">TTL_State (I rename this setting 'color' and name the TTL states that</div>
<div style="font-family: Consolas;">result in the desired outputs 'green', 'red' and 'off'. This lets me set</div>
<div style="font-family: Consolas;">multi-channel images by assigning the 'color' variable in Multi-D</div>
<div style="font-family: Consolas;">Acquisition)</div>
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<div style="font-family: Consolas;">Turn on DigitalModulation in the Device Property Browser and you should be</div>
<div style="font-family: Consolas;">set. You have to switch LaserEmission on each time you boot up MM and if</div>
<div style="font-family: Consolas;">you use a TTL controller PeakPower will determine the laser intensity.</div>
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<div style="font-family: Consolas;">Blanking mode will theoretically help acquire faster with minimized light</div>
<div style="font-family: Consolas;">exposure. However I have not got that to work; Micro-Manager's</div>
<div style="font-family: Consolas;">auto-shutter seems to do the job well enough.</div>
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<div style="font-family: Consolas;">All the best, </div>
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<div style="font-family: Consolas;">Tim</div>
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<div style="font-family: Consolas;">Timothy Feinstein, Ph.D.</div>
<div style="font-family: Consolas;">Research Scientist</div>
<div style="font-family: Consolas;">University of Pittsburgh Department of Developmental Biology</div>
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<span style="font-weight:bold">From: </span>OpenSPIM <<a href="mailto:openspim-bounces@openspim.org">openspim-bounces@openspim.org</a>> on behalf of "Weiss, Kurt" <<a href="mailto:KWeiss@morgridge.org">KWeiss@morgridge.org</a>><br>
<span style="font-weight:bold">Date: </span>Monday, December 5, 2016 at 5:22 PM<br>
<span style="font-weight:bold">To: </span>"<a href="mailto:openspim@openspim.org">openspim@openspim.org</a>" <<a href="mailto:openspim@openspim.org">openspim@openspim.org</a>><br>
<span style="font-weight:bold">Subject: </span>[OpenSPIM] mutli-channel OpenSPIM<br>
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<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;">Hi,
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<div>We are interested in adding more laser channels to our current OpenSPIM. There seem to be three options: (1) multi-channel lauch, (2) laser combiner unit, (3) combine with mirror + dichroic. </div>
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<div><span style="white-space: pre-wrap; font-size: 13.3333px;">If we go for option 1: Does anyone have experience with mult-channel modules which integrate well with the OpenSPIM platform? </span><span style="font-size: 13.3333px; white-space: pre-wrap;">(Besides
the Stradus Versalase I have seen mentioned on this forum, though updates on this unit might be helpful if anyone has strong feelings).</span></div>
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<div><span style="white-space: pre-wrap; font-size: 13.3333px;">Any other comments on options 2 or 3 would also be appreciated. A few concerns have been addressed here:</span></div>
<div><span style="font-size: 13.3333px; white-space: pre-wrap;"><a href="http://openspim.org/pipermail/openspim/2013-December/001268.html">http://openspim.org/pipermail/openspim/2013-December/001268.html</a></span></div>
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<div><span style="white-space: pre-wrap; font-size: 10pt;">Thanks, </span></div>
<div><span style="white-space: pre-wrap; font-size: 10pt;">Kurt </span></div>
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<div style="font-family:Tahoma; font-size:13px"><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size: small; background-color: rgb(255, 255, 255);">_______________________</span><br style="color:rgb(34,34,34); font-family:arial,sans-serif; font-size:small">
<span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size: small;">Kurt</span><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size: small; background-color: rgb(255, 255, 255);"> </span><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size: small;">Weiss, </span><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size: small; background-color: rgb(255, 255, 255);">Ph.D.</span>
<div style="color:rgb(34,34,34); font-family:arial,sans-serif; font-size:small">Postdoctoral Associate<br>
<div style="color:rgb(0,0,0); font-family:tahoma; font-size:13px">Huisken Group, Medical Engineering</div>
<div style="color:rgb(0,0,0); font-family:tahoma; font-size:13px">Morgridge Institute for Research</div>
<div style="color:rgb(0,0,0); font-family:tahoma; font-size:13px">330 N Charter St. </div>
<div style="color:rgb(0,0,0); font-family:tahoma; font-size:13px">Madison, WI 53715</div>
<div style="color:rgb(0,0,0); font-family:tahoma; font-size:13px"><a href="mailto:KWeiss@morgridge.org">KWeiss@morgridge.org</a></div>
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