[OpenSPIM] Can you tell me the possible reasons for registration failure

[Pavel Tomancak]

Dear Zheng,

This could mean several things, for example that you do not have enough beads around the sample, do not segment them properly or that your views overlap only minimally and thus do not share enough beads. Another reason might be that you did not enter correct calibration, i.e. the ratio between xy and z dimensions. If that is the case the registration cannot work.

I recommend to read the paper about bead based registration and the documentation on the Fiji wiki [1] to understand what the algorithm is doing and what these reported numbers mean. These are not error messages, they are meant to help you understand where lies the problem in your registration. 

All the best

PAvel

[1] http://fiji.sc/SPIM_Registration_Method
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Pavel Tomancak, Ph.D.

Research Group Leader
Max Planck Institute of Molecular Cell Biology and Genetics in Dresden
Pfotenhauerstr. 108
D-01307 Dresden							Tel.: +49 351 210 2670
Germany									Fax: +49 351 210 2020

tomancak at mpi-cbg.de
http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html
twitter: @PavelTomancak
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On Sep 14, 2015, at 4:48 AM, 政 <709314090 at qq.com> wrote:

> Hi, everyone,
>     Sorry to disturb you again. I try my best to do the registration work, but it didn't work. It still alerts something follow
> spim_TL01_Angle2.tif<->spim_TL01_Angle4.tif: Not enough correspondences found 6, should be at least 12
> spim_TL01_Angle2.tif<->spim_TL01_Angle3.tif: Model found but not enough remaining inliers (4/12) after RANSAC of 12
> 
>     Can someone tell me some possible reasons whick would lead to these errors? Both in the data process and experiment.
> 
>     Thank you very much!
> 
> All best!
> ------------------
> Zheng Huang
> State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China
> Address: Qinghe Road No. 390, Jiading District, Shanghai 201800, China 
> Tel: 13120836853
> E-mail: 709314090 at qq.com
>  
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