[OpenSPIM] pinhole location and diameter to clean up the laser beam

Claire Lionnet claire.lionnet at ens-lyon.fr
Mon Sep 14 04:48:15 CDT 2015


Hi everyone,

We've got an openspim almost ready to work here in France. We are just 
concerned by the not-very-circular laser beam. Did somebody used a 
pinhole to clean up the beam ? What would be the best location for a 
pinhole in this setup : in the first or the second telescope ? And what 
should be its diameter ? (sorry for the basic questions, not an optical 
engineer !).
Thanks in advance for your help,

Claire

-- 
Claire LIONNET
PLATIM
US8 / UMS3444 BioSciences Gerland - Lyon Sud
http://www.sfr-biosciences.fr/plateformes/Phenotypage-cellulaire/PLATIM






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