[OpenSPIM] changing "objectives"

[Fredrik Ek]

Hi Monika,

Please see the attached picture. We have from the imaging chamber tube, filterwheel, tube, tubelens, magnifier and finally camera.

Regards

Fredrik

-----Ursprungligt meddelande-----
Från: openspim-bounces at openspim.org [mailto:openspim-bounces at openspim.org] För Monika Pawlowska
Skickat: den 28 oktober 2015 11:01
Till: openspim
Ämne: Re: [OpenSPIM] changing "objectives"

Hi Peter & others,

it's a stupid question, but could you clarify how the magnifier is installed? In the basic design it is: detection objective-distance-tube lens-distance-camera. And with the magnifier? Is it used instead of the tube lens or additionally?

Regards,
Monika


W dniu .09.2015 o 08:56 Peter Gabriel Pitrone <pitrone at mpi-cbg.de> pisze:

> Hello Timothy,
>
> We use the U-CA to much success... Aligning the light sheet for each 
> mag is highly recommended. I've even use the 0.5x C-mount with it, but 
> that doesn't work with large chip detectors. Another minor issue I 
> have with it is it's range, it only doubles the mag over 4 slots 
> (Empty, 1.25x, 1.6x, & 2.0x).
>
> Regards,
> Pete
>
> Peter Gabriel Pitrone DipRMS
> Microscopy and Imaging specialist
> in the Dr. Pavel Tomancak group at
> the Max Planck Institute of Molecular
> Cell Biology and Genetics, Dresden
> Pfotenhauerstrasse 108
> 01307 Dresden
> Germany
>
>> On Sep 22, 2015, at 5:34 PM, Feinstein, Timothy N <tnf8 at pitt.edu> wrote:
>>
>> Hi folks,
>>
>> Thanks to the indispensable help of many users here, our SPIM is up 
>> and working.  You are all the best.
>>
>> Since a SPIM makes it quite hard to change objectives, I bought 1x 
>> and a 0.5x C-mount camera adapters to get the equivalent of a 20x and 
>> a 10x objective.  It seems to work quite well.  Now I wonder whether 
>> anyone has tried a magnifying tube lens like the Olympus U-CA or 
>> U-ECA to get a higher power image.  It all depends on whether a 
>> 'vanilla' openSPIM design is already imaging near the Nyquist limit.  
>> We get 0.33 um/px using the Olympus 20x/0.5 and a Hamamatsu Flash4.0 v2, and even with
>> deconvolution it may be pointless to magnify the image any further.   
>> Any thoughts appreciated.
>>
>> Alternatively, has anyone tried keeping a second sample chamber with 
>> a higher power objective to swap in as needed?  It seems like a pain 
>> but less cumbersome than building a second instrument.
>>
>> Thanks,
>>
>>
>> Tim
>>
>> Timothy Feinstein, Ph.D.
>> Research Scientist
>> University of Pittsburgh Department of Developmental Biology
>>
>>
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>
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--
Dr Monika Pawłowska
Nencki Institute
02-093 Warsaw
Pasteura 3
Poland

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