[OpenSPIM] changing "objectives"

[Monika Pawłowska]

Hi Peter & others,

it's a stupid question, but could you clarify how the magnifier is  
installed? In the basic design it is: detection objective-distance-tube  
lens-distance-camera. And with the magnifier? Is it used instead of the  
tube lens or additionally?

Regards,
Monika


W dniu .09.2015 o 08:56 Peter Gabriel Pitrone <pitrone at mpi-cbg.de> pisze:

> Hello Timothy,
>
> We use the U-CA to much success... Aligning the light sheet for each mag  
> is highly recommended. I've even use the 0.5x C-mount with it, but that  
> doesn't work with large chip detectors. Another minor issue I have with  
> it is it's range, it only doubles the mag over 4 slots (Empty, 1.25x,  
> 1.6x, & 2.0x).
>
> Regards,
> Pete
>
> Peter Gabriel Pitrone DipRMS
> Microscopy and Imaging specialist
> in the Dr. Pavel Tomancak group at
> the Max Planck Institute of Molecular
> Cell Biology and Genetics, Dresden
> Pfotenhauerstrasse 108
> 01307 Dresden
> Germany
>
>> On Sep 22, 2015, at 5:34 PM, Feinstein, Timothy N <tnf8 at pitt.edu> wrote:
>>
>> Hi folks,
>>
>> Thanks to the indispensable help of many users here, our SPIM is up and  
>> working.  You are all the best.
>>
>> Since a SPIM makes it quite hard to change objectives, I bought 1x and  
>> a 0.5x C-mount camera adapters to get the equivalent of a 20x and a 10x  
>> objective.  It seems to work quite well.  Now I wonder whether anyone  
>> has tried a magnifying tube lens like the Olympus U-CA or U-ECA to get  
>> a higher power image.  It all depends on whether a 'vanilla' openSPIM  
>> design is already imaging near the Nyquist limit.  We get 0.33 um/px  
>> using the Olympus 20x/0.5 and a Hamamatsu Flash4.0 v2, and even with  
>> deconvolution it may be pointless to magnify the image any further.   
>> Any thoughts appreciated.
>>
>> Alternatively, has anyone tried keeping a second sample chamber with a  
>> higher power objective to swap in as needed?  It seems like a pain but  
>> less cumbersome than building a second instrument.
>>
>> Thanks,
>>
>>
>> Tim
>>
>> Timothy Feinstein, Ph.D.
>> Research Scientist
>> University of Pittsburgh Department of Developmental Biology
>>
>>
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-- 
Dr Monika Pawłowska
Nencki Institute
02-093 Warsaw
Pasteura 3
Poland