[OpenSPIM] OpenSPIM and optically cleared 3D tissue

[Jamie Flynn]

Hi everyone,

We're building a dual illumination OpenSPIM at the Hunter Medical Research Institute in Newcastle, Australia. Our aim is to have the scope and analysis pipeline used as a core facility for lab groups to image 3D tumour cell cultures, developing embryos and most importantly for us, optically cleared 3D tissue samples (e.g. CLARITY, CUBIC, 3DISCO, Scale etc.). Imaging cleared 3D samples would be an interesting avenue for OpenSPIM and it'd be great to hear about any hardware modifications or mounting protocols others may have used to do it.

If anyone wants to try clearing out their own tissue samples, my colleagues and I put together a handbook that describes the reagents, equipment and detailed protocols for 'Passive CLARITY'. We compiled the protocols outlined in Tomer et al. Nat. Prot. 9(7) 2014 and Yang et al. Cell (158) 2014 and added in our own refinements (after much trial and error!). It's a very simple technique and has worked really well in our hands. Here's the link. Download it to your desktop in case the link gets broken:

https://drive.google.com/open?id=0BzIZDgZFR2srNFk2dHFqT1UycFk

All the best
--------------------
Jamie Flynn (PhD)
University of Newcastle
Hunter Medical Research Institute
Room 3613 level 3 east
1 Kookaburra Circuit, New Lambton Heights
NSW 2305, Australia.
pH: +612 40420468
Mobile: +61 416003787
Email: jamie.flynn at uon.edu.au

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