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<div style="font-size: 14px; font-family: Calibri, sans-serif;">Hi everyone,</div>
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<div><font face="Calibri,sans-serif" style="font-size: 14px;">We’re building a dual illumination OpenSPIM at the Hunter Medical Research Institute in Newcastle, Australia. Our ai</font>m is to have the scope and analysis pipeline used as a core facility for
lab groups to image 3D tumour cell cultures, developing embryos and most importantly for us, optically cleared 3D tissue samples (e.g. CLARITY, CUBIC, 3DISCO, Scale etc.). Imaging cleared 3D samples would be an interesting avenue for OpenSPIM and it’d be great
to hear about any hardware modifications or mounting protocols others may have used to do it.</div>
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<div>If anyone wants to try clearing out their own tissue samples, my colleagues and I put together a handbook that describes the reagents, equipment and detailed protocols for ‘Passive CLARITY’. We compiled the protocols outlined in <i>Tomer et al. Nat. Prot.
9(7) 2014 </i>and <span style="font-style: italic;">Yang et al. Cell (158) 2014 </span>
and added in our own refinements (after much trial and error!). It’s a very simple technique and has worked really well in our hands. Here’s the link. Download it to your desktop in case the link gets broken:</div>
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<div style="font-size: 14px; font-family: Calibri, sans-serif;"><a href="https://drive.google.com/open?id=0BzIZDgZFR2srNFk2dHFqT1UycFk">https://drive.google.com/open?id=0BzIZDgZFR2srNFk2dHFqT1UycFk</a></div>
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<div style="font-size: 14px; font-family: Calibri, sans-serif;">All the best</div>
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<div>Jamie Flynn (PhD)</div>
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<div>University of Newcastle</div>
<div>Hunter Medical Research Institute</div>
<div>Room 3613 level 3 east</div>
<div><span style="widows: 1; background-color: rgb(255, 255, 255);">1 Kookaburra Circuit, New Lambton Heights</span></div>
<div><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size: 13px; line-height: 16.1200008392334px; widows: 1; background-color: rgb(255, 255, 255);">NSW 2305, Australia.</span></div>
<div><span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size: 13px; line-height: 16.1200008392334px; widows: 1; background-color: rgb(255, 254, 254);">pH: +612 40420468</span></div>
<div>Mobile: <span style="color: rgb(34, 34, 34); font-family: arial, sans-serif; font-size: 13px; line-height: 16.1200008392334px; widows: 1; background-color: rgb(255, 254, 254);">+61 416003787</span></div>
<div>Email: jamie.flynn@uon.edu.au</div>
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