[OpenSPIM] Image Stitching Best Practices

[m.pawlowska at nencki.gov.pl]

Hi Gabriel,

I was struggling a bit with the stitching step. In the end what worked 
for me is:
first convert to tif using modified script from here:
http://openspim.org/Pre-processing

then use Grid&collection stitching with settings: type=[Grid: 
column-by-column] order=[Down & Right]

It seems stupid to convert images to tiff and lose the metadata 
contained in ome, but I get errors otherwise. :/ If anybody has a better 
procedure, maybe they could describe it?

Regards,
Monika



W dniu 2015-06-27 07:18, Gabriel Davis Jones napisał(a):
> Hi All,
> 
> We are currently imaging large volumes that require us to acquire
> multiple stacks of the same angle.
> Is there currently a best way to stitch images acquired OpenSPIM?
> Should we use the grid/collection stitching plugin or is there a
> better way to go about it?
> 
> Thanks,
> 
> Gabriel
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