[OpenSPIM] Choice of emission filters

[brau]

Dear open spim community members,

We have built an open spim in our MICA facility but have problem of 
reflexions (transmission of the 488nm laser) in the emission path. We 
use a quite good Brightline 525/50 band pass filter from Semrock. This 
transmission of the 488nm is due to the dependance of the wavelength 
transmission of the filter with the angle of incidence. From your 
practical experience, what filter, or combination or filters, could we 
put or add to avoid this problem?
Thanks in advance,
Best regards,
Frédéric

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Frédéric Brau
Institut de Pharmacologie Moléculaire et Cellulaire - UMR7275 CNRS/UNS
660, Route des Lucioles
06560 VALBONNE
Courriel : brau at ipmc.cnrs.fr
Web : http://www.ipmc.cnrs.fr/
Tel: 33 (0)4 93 95 77 83 Bureau / 77 87 / 77 88 / 77 89 Confocaux
Fax: 33 (0)4 93 95 77 08
Plateforme Microscopie Imagerie Côte d'Azur : http://www.mica-bio.fr
Réseau Technologique de Microscopie de Fluorescence Multidimensionnelle 
: http://rtmfm.cnrs.fr/
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