[OpenSPIM] Input data for SPIM Registration PlugIn

[Kieran Short]

Richard,

The endianness of the TIFF data might be wrong (wrong byte order). Try
switching to intel-byte order (or if it already is, switch it to big
endian).

fyi: http://en.wikipedia.org/wiki/Endianness

cheers,
Kieran


On Tue, May 27, 2014 at 8:19 PM, <Shugi at gmx.de> wrote:

> Hello,
>
>  I read about your tool for registration and fusion of multiple view
> images. It is the topic of my intership to find a way doing this with our
> data and I would like to try your tool. But it seems to have a problem with
> our data. The original data from a Z1 are present as czi file. I extract
> them as ome.tif file with the ZEN2012 Software. Like in the tutorial the
> extension was changed from ome.tif to tif. I was following the tutorial and
> when to interactivlly choosing the threshold for the beads and click "Done"
> the following Messages occure in the Log:
>
> Opening first image to determine z-stretching.
> Cannot open fie: ...
>
> The path and the filename are correct. Maybe it is something about the
> formate? Do you need special tags in the tiff file or does the bit depth
> has influence?
> Hope you can help me, because your tool seems to be very promising.
>
> Kind regardas
> Ricarda Bräuniger
>
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