[OpenSPIM] Welcome to the OpenSPIM community, Kumar!

[Krieger, Jan]

Hi Kumar!

we are using a SPIM to image single mamallian cells, which is already close to applications in bacteria. We use imaging FCS inside the cells. For use the modification of the SPIM was not too hard: basically you need a thin lightsheet (~1-2µm), which sections the cell and you need to mount the cells. There are several ways of achieving the latter: Either you embed them in a gel, or if you have adherent cells, you can simply grow them on a piece of coverslip, which is then mounted in the sample chamber. Here are some refs, which might be interesting for you:
http://www.nature.com/nbt/journal/v29/n9/full/nbt.1928.html
http://www.opticsinfobase.org/abstract.cfm?URI=oe-22-3-2358
http://mcb.asm.org/cgi/doi/10.1128/MCB.01097-13
http://www.nature.com/nmeth/journal/v8/n5/full/nmeth.1586.html
http://doi.wiley.com/10.1111/jmi.12051
http://www.celldiv.com/content/6/1/22
http://dx.doi.org/10.1186%2F1747-1028-6-22  
http://www.nature.com/nmeth/journal/v10/n5/full/nmeth.2411.html?WT.ec_id=NMETH-201305
http://dx.doi.org/10.1371%2Fjournal.pone.0050846

I hope that helps ;-) ... BTW: As you are in Singapore, maybe talk to Anand Prataph Singh who did his PhD in the group of Thorsten Wohland at NUS.

Best,
JAN


Dipl.-Phys. Jan Krieger
German Cancer Research Center (dkfz)
Department B040 - Biophysics of Macromolecules (Prof. J. Langowski)
Im Neuenheimer Feld 580
69120 Heidelberg

fon: +49 / 6221 / 42-3395
fax: +49 / 6221 / 42-3391
e-mail: j.krieger at dkfz.de
www: http://www.dkfz.de/Macromol/
________________________________________
Von: openspim-bounces at openspim.org [openspim-bounces at openspim.org] im Auftrag von #KUMARAVEL KANDASWAMY# [KUMARAVE001 at e.ntu.edu.sg]
Gesendet: Dienstag, 18. März 2014 16:49
An: Johannes Schindelin
Cc: openspim at openspim.org
Betreff: Re: [OpenSPIM] Welcome to the OpenSPIM community, Kumar!

Hi Johannes.

Thanks for the note, I wasn't aware of this before.  Please move the post to the appropriate destination.

Just wanted to know if anyone has tried SPIM for studying developmental biology of bacteria. I would be pleased to know the SPIM modifications required for Imaging bacteria. Such a modifications in the sample chamber/carriers.

Thank you,

Best regards
Kumar


-----Original Message-----
From: Johannes Schindelin [mailto:Johannes.Schindelin at gmx.de]
Sent: Tuesday, 18 March 2014 11:34 PM
To: #KUMARAVEL KANDASWAMY#
Cc: openspim at openspim.org
Subject: Welcome to the OpenSPIM community, Kumar!

Hi Kumar,

you posted a request on the OpenSPIM Wiki, but we do not use the Wiki as a forum. Instead, we use the OpenSPIM mailing list (Cc:ed). Let's move the conversation to the list?

Ciao,
Johannes

-- Kumar's message --
Articles for SPIM implementation for Biofilms

Hi all

I am Kumar from SCELSE(NTU) Singapore. I am here to follow the SPIM implementation for biofilms exclusively for bacterial communities.

I would love to see more articles discussing on several advances in this field. specifically on SPIM modifications,fluorescent probes, sample carriers and image analysis for bacterial imaging.

Here is one http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-10-2-145

Happy reading

Cheers

Kumar

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