[OpenSPIM] Picard Stage Adapter problem (Luke Stuyvenberg) and light sheet thickness

[Aurelia Honerkamp-Smith]

Hello,

Thanks for this latest update: it has almost fixed my PVCAM problem.  Now I
can load the camera, and since the last update I no longer get a time-out
error message when I use the live view.  But, the live window opens and
displays an image, then it doesn't refresh, so I see the same image until I
stop and start live view again.  I can now run the Open SPIM plugin, but
have to keep stopping and starting live view in order to see what is going
on with my calibration sample.  I'm so close!  Any suggestions for fixing
this?  Live view through my camera works fine in a plain, non-Fiji and
non-OpenSPIM installation of Micromanager, so I can align and take pictures
at the moment, but I want to use your lovely plugin.

And another thing... I remember some discussion from earlier this summer
about optimizing the optics.  I have made a light sheet that's about 10
microns thick, minimizing its thickness by adjusting the second telescope
lens as described on the website.  Can I get it thinner than that?  What
should I move to do so?

Thanks again,

Aurelia



>
>
> -----Luke Stuyvenberg <stuyvenberg at wisc.edu> schrieb: -----
> An: edgar.escobar.nieto at ipt.fraunhofer.de, Johannes Schindelin <
> Johannes.Schindelin at gmx.de>
> Von: Luke Stuyvenberg <stuyvenberg at wisc.edu>
> Datum: 26.09.2013 00:21
> Kopie: openspim at openspim.org
> Betreff: Re: [OpenSPIM] WG: Re:  Picard Stage Adapter problem
>
> Hi Edgar and everyone,
>
> With regards to the DLL problems that recent updates have generated:
> - It seems I accidentally built PicardStage in debug mode (hence the 'D'
> on the end of the DLL names it became dependent on) -- I've since fixed
> that issue, uploaded the correct version of the DLL, and taken steps to
> ensure it won't happen again.
> - The troubles with TIScam (and some other device adapters) was an
> artifact of our Visual Studio upgrade: the device adapters became dependent
> on the '100' (VS2010) versions of the files. Since Micro-Manager does not
> yet come bundled with the VS2010 runtime environment, most users will be
> missing those files. I've fixed these issues and uploaded correct versions
> to the update site as well.
> If you are having trouble with device adapters not loading, please try
> updating through Fiji again and let me know if the problems persist.
>
>
> On 09/25/13, edgar.escobar.nieto at ipt.fraunhofer.de wrote:
> > I just downloaded this files from internet and copied to
> C:\WINDOWS\system32 and now I am able to use the Picard Stage
> > using the updated version of Fiji.
> I strongly recommend against downloading and installing DLLs from the
> internet unless they are from the authoritative source (in the case of
> these 'MSVC' files, Microsoft) -- I would suggest instead downloading the
> Microsoft Visual C++ Redistributable Package --?
> http://www.microsoft.com/en-us/download/details.aspx?id=5555?for 32-bit
> computers?-- which contains the necessary files.
>
>
> Sorry about all the trouble! If there are any more problems, please e-mail
> Johannes, myself, and/or the list.
>
>
> Thanks,
> Luke Stuyvenberg
>
>
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