<div dir="ltr"><div>Hello, <br><br>Thanks for this latest update: it has almost fixed my PVCAM problem. Now I can load the camera, and since the last update I no longer get a time-out error message when I use the live view. But, the live window opens and displays an image, then it doesn't refresh, so I see the same image until I stop and start live view again. I can now run the Open SPIM plugin, but have to keep stopping and starting live view in order to see what is going on with my calibration sample. I'm so close! Any suggestions for fixing this? Live view through my camera works fine in a plain, non-Fiji and non-OpenSPIM installation of Micromanager, so I can align and take pictures at the moment, but I want to use your lovely plugin. <br>
<br></div><div>And another thing... I remember some discussion from earlier this summer about optimizing the optics. I have made a light sheet that's about 10 microns thick, minimizing its thickness by adjusting the second telescope lens as described on the website. Can I get it thinner than that? What should I move to do so? <br>
<br></div><div>Thanks again, <br><br>Aurelia<br><br> </div><div><div><div class="gmail_extra"><br><div class="gmail_quote"><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
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-----Luke Stuyvenberg <<a href="mailto:stuyvenberg@wisc.edu">stuyvenberg@wisc.edu</a>> schrieb: -----<br>
An: <a href="mailto:edgar.escobar.nieto@ipt.fraunhofer.de">edgar.escobar.nieto@ipt.fraunhofer.de</a>, Johannes Schindelin <<a href="mailto:Johannes.Schindelin@gmx.de">Johannes.Schindelin@gmx.de</a>><br>
Von: Luke Stuyvenberg <<a href="mailto:stuyvenberg@wisc.edu">stuyvenberg@wisc.edu</a>><br>
Datum: <a href="tel:26.09.2013%2000" value="+12609201300">26.09.2013 00</a>:21<br>
Kopie: <a href="mailto:openspim@openspim.org">openspim@openspim.org</a><br>
Betreff: Re: [OpenSPIM] WG: Re: Picard Stage Adapter problem<br>
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Hi Edgar and everyone,<br>
<br>
With regards to the DLL problems that recent updates have generated:<br>
- It seems I accidentally built PicardStage in debug mode (hence the 'D' on the end of the DLL names it became dependent on) -- I've since fixed that issue, uploaded the correct version of the DLL, and taken steps to ensure it won't happen again.<br>
- The troubles with TIScam (and some other device adapters) was an artifact of our Visual Studio upgrade: the device adapters became dependent on the '100' (VS2010) versions of the files. Since Micro-Manager does not yet come bundled with the VS2010 runtime environment, most users will be missing those files. I've fixed these issues and uploaded correct versions to the update site as well.<br>
If you are having trouble with device adapters not loading, please try updating through Fiji again and let me know if the problems persist.<br>
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On 09/25/13, <a href="mailto:edgar.escobar.nieto@ipt.fraunhofer.de">edgar.escobar.nieto@ipt.fraunhofer.de</a> wrote:<br>
> I just downloaded this files from internet and copied to C:\WINDOWS\system32 and now I am able to use the Picard Stage<br>
> using the updated version of Fiji.<br>
I strongly recommend against downloading and installing DLLs from the internet unless they are from the authoritative source (in the case of these 'MSVC' files, Microsoft) -- I would suggest instead downloading the Microsoft Visual C++ Redistributable Package --?<a href="http://www.microsoft.com/en-us/download/details.aspx?id=5555?for" target="_blank">http://www.microsoft.com/en-us/download/details.aspx?id=5555?for</a> 32-bit computers?-- which contains the necessary files.<br>
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Sorry about all the trouble! If there are any more problems, please e-mail Johannes, myself, and/or the list.<br>
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Thanks,<br>
Luke Stuyvenberg<br>
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