[OpenSPIM] lasers for SPIM

[Michael Weber]

Hi Jan,

we are using Coherent Sapphire DPSS lasers in some of our setups and cannot complain about the beam quality or spectral properties. The disadvantage is that for best convenience you need an AOTF which you have to buy and align, but you can also go for ND filters once you figured out how much power you really need. As Pete mentioned, there are issues with diode lasers in terms of spectral properties (green background from a 488 nm laser, requires clean up filter), but you can nicely control them over USB. They also tend to be more compact. You will probably end up with a cheaper and more compact system if you go for diode lasers, and with a slightly better performance if you go for DPSS lasers.

Best,
Michael

On Sep 2, 2013, at 11:11 AM, Peter Gabriel Pitrone <pitrone at mpi-cbg.de> wrote:

> 
> Hello Jan,
> 
> We have used both the Coherent CUBE and the OBIS (488ish nm, 50 mW) on
> our system, and they work really well... BUT Diode lasers such as these
> really do need a clean up filter, as there is a (unfortunately
> unmeasurable) bit of parasitic green light in the range of the emission
> of GFP/YFP.
> 
> Maybe someone from the Huisken lab can chime in to tell you about the use
> of the Sapphire, as it does not have the same characteristics as the CUB
> or OBIS.
> 
> Best regards,
> 
> Pete
> 
> -- 
> Peter Gabriel Pitrone - TechRMS
> Microscopy/Imaging Specialist
> Prof. Dr. Pavel Tomancak group
> Max Planck Institute for
> Molecular Biology and Genetics
> Pfotenhauerstr. 108
> 01307 Dresden
> 
> "If a straight line fit is required, obtain only two data points." - Anon.
> 
> 
> On Sat, August 31, 2013 9:22 am, Krieger, Jan wrote:
> <|> Hi everybody!
> <|>
> <|> as one of our lasers (491nm) died down yesterday, I wanted to ask, which
> <|> lasers you prefer for the openSPIM:
> <|>
> <|> - So we use Cobolt DPSS lasers, which can not be modulated
> <|> - If we can't repair our laser, I thought about getting one of these:
> <|>    1. a Cobolt MLD http://www.cobolt.se/coboltmld.html
> <|>    2. Coherent Cube http://www.coherent.com/products/?1007/CUBE
> <|>    3. Coherent Saphire
> <|> http://www.coherent.com/products/?1638/Sapphire-Lasers
> <|>    4. Coherent OBIS http://www.coherent.com/products/?1884/OBIS-Lasers
> <|>
> <|> I would appreciate any help/hints you can give me on these (and possibly
> <|> other) lasers, that you think suitable for SPIM.
> <|>
> <|> Best,
> <|> JAN
> <|>
> <|>
> <|> Dipl.-Phys. Jan Krieger
> <|> German Cancer Research Center (dkfz)
> <|> Department B040 - Biophysics of Macromolecules (Prof. J. Langowski)
> <|> Im Neuenheimer Feld 580
> <|> 69120 Heidelberg
> <|>
> <|> fon: +49 / 6221 / 42-3395
> <|> fax: +49 / 6221 / 42-3391
> <|> e-mail: j.krieger at dkfz.de
> <|> www: http://www.dkfz.de/Macromol/
> <|> _______________________________________________
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> <|> OpenSPIM at openspim.org
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> <|>
> 
> 
> 
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_____________

Michael Weber
PhD Student, Huisken lab
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108, 01307 Dresden
Tel. 0049 351/2102837

http://www.mpi-cbg.de/huisken

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