[OpenSPIM] lasers for SPIM

[Peter Gabriel Pitrone]

Hello Jan,

We have used both the Coherent CUBE and the OBIS (488ish nm, 50 mW) on
our system, and they work really well... BUT Diode lasers such as these
really do need a clean up filter, as there is a (unfortunately
unmeasurable) bit of parasitic green light in the range of the emission
of GFP/YFP.

Maybe someone from the Huisken lab can chime in to tell you about the use
of the Sapphire, as it does not have the same characteristics as the CUB
or OBIS.

Best regards,

Pete

-- 
Peter Gabriel Pitrone - TechRMS
Microscopy/Imaging Specialist
Prof. Dr. Pavel Tomancak group
Max Planck Institute for
Molecular Biology and Genetics
Pfotenhauerstr. 108
01307 Dresden

"If a straight line fit is required, obtain only two data points." - Anon.


On Sat, August 31, 2013 9:22 am, Krieger, Jan wrote:
<|> Hi everybody!
<|>
<|> as one of our lasers (491nm) died down yesterday, I wanted to ask, which
<|> lasers you prefer for the openSPIM:
<|>
<|> - So we use Cobolt DPSS lasers, which can not be modulated
<|> - If we can't repair our laser, I thought about getting one of these:
<|>    1. a Cobolt MLD http://www.cobolt.se/coboltmld.html
<|>    2. Coherent Cube http://www.coherent.com/products/?1007/CUBE
<|>    3. Coherent Saphire
<|> http://www.coherent.com/products/?1638/Sapphire-Lasers
<|>    4. Coherent OBIS http://www.coherent.com/products/?1884/OBIS-Lasers
<|>
<|> I would appreciate any help/hints you can give me on these (and possibly
<|> other) lasers, that you think suitable for SPIM.
<|>
<|> Best,
<|> JAN
<|>
<|>
<|> Dipl.-Phys. Jan Krieger
<|> German Cancer Research Center (dkfz)
<|> Department B040 - Biophysics of Macromolecules (Prof. J. Langowski)
<|> Im Neuenheimer Feld 580
<|> 69120 Heidelberg
<|>
<|> fon: +49 / 6221 / 42-3395
<|> fax: +49 / 6221 / 42-3391
<|> e-mail: j.krieger at dkfz.de
<|> www: http://www.dkfz.de/Macromol/
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<|>