[OpenSPIM] Request for information on open SPIM

[Jan Huisken]

Dear Kumar,

we have a paper on zebrafish mounting for SPIM with FEP tubes:
http://www.ncbi.nlm.nih.gov/pubmed/22872089

Best
Jan
 
Dr. Jan Huisken
MPI of Molecular Cell Biology and Genetics
Pfotenhauerstr. 108, 01307 Dresden, Germany

On Oct 6, 2013, at 10:40 AM, Pavel Tomancak wrote:

> Forwarding… Do we have a wiki page on the FEP tubes?
> 
> PAvel
> 
>> From: #KUMARAVEL KANDASWAMY# <KUMARAVE001 at e.ntu.edu.sg>
>> Subject: Request for information on open SPIM
>> Date: October 5, 2013 8:33:19 PM GMT+02:00
>> To: "tomancak at mpi-cbg.de" <tomancak at mpi-cbg.de>
>> 
>> Dear Dr. Paval,
>>  
>> I am Kumar, Graduate student from NTU-Singapore. I referred to your recent article on open SPIM (http://www.nature.com/nmeth/journal/v10/n7/full/nmeth.2507.html#videos) and I find this technique is amazing and I would like to try this method to study developmental biology of bacterial biofilms with reporter strains. I am currently trying this with the custom built SPIM system however I am quite unclear with the carriers for sample fixation, I would like to seek your advice on this.
>>  
>> It would be really helpful if you could advise me on the sample preparation techniques for SPIM, if you are using any FEP tubing’s to immobilize the sample kindly advise me on the specifications(i,e tubing material, dimensions and refractive index)  of the tubing’s.
>>  
>> Looking forward to your reply,
>>  
>> Thank you and have a great day.
>>  
>> Best regards
>> Kumar
>>  
>> <image001.gif>
>> Kumaravel Kandaswamy | Research Student| Nanyang Technological University
>> Singapore Centre on Environmental Life Sciences Engineering (SCELSE)
>> SBS-B2N-27, 60 Nanyang Drive, Singapore 637551
>> Tel: (65) 82649175 | Fax: (65) 65156751 | Email: kumarave001 at e.ntu.edu.sg |Web: www.scelse.sg
>>  
>>  
> 
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