<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Dear Kumar,<div><br></div><div>we have a paper on zebrafish mounting for SPIM with FEP tubes:</div><div><a href="http://www.ncbi.nlm.nih.gov/pubmed/22872089">http://www.ncbi.nlm.nih.gov/pubmed/22872089</a></div><div><br></div><div>Best</div><div>Jan</div><div> <br><div apple-content-edited="true">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div style="font-size: 11px; ">Dr. Jan Huisken</div><div style="font-size: 11px; "><b>MPI of Molecular Cell Biology and Genetics</b></div><div style="font-size: 11px; ">Pfotenhauerstr. 108, 01307 Dresden, Germany</div></div></span></div>
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<br><div><div>On Oct 6, 2013, at 10:40 AM, Pavel Tomancak wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><meta http-equiv="Content-Type" content="text/html charset=windows-1252"><base href="x-msg://535/"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Forwarding� Do we have a wiki page on the FEP tubes?<div><br></div><div>PAvel<br><div><div><br class="Apple-interchange-newline"><blockquote type="cite"><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;"><span style="font-family:'Helvetica'; font-size:medium; color:rgba(0, 0, 0, 1.0);"><b>From: </b></span><span style="font-family:'Helvetica'; font-size:medium;">#KUMARAVEL KANDASWAMY# <<a href="mailto:KUMARAVE001@e.ntu.edu.sg">KUMARAVE001@e.ntu.edu.sg</a>><br></span></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;"><span style="font-family:'Helvetica'; font-size:medium; color:rgba(0, 0, 0, 1.0);"><b>Subject: </b></span><span style="font-family:'Helvetica'; font-size:medium;"><b>Request for information on open SPIM</b><br></span></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;"><span style="font-family:'Helvetica'; font-size:medium; color:rgba(0, 0, 0, 1.0);"><b>Date: </b></span><span style="font-family:'Helvetica'; font-size:medium;">October 5, 2013 8:33:19 PM GMT+02:00<br></span></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;"><span style="font-family:'Helvetica'; font-size:medium; color:rgba(0, 0, 0, 1.0);"><b>To: </b></span><span style="font-family:'Helvetica'; font-size:medium;">"<a href="mailto:tomancak@mpi-cbg.de">tomancak@mpi-cbg.de</a>" <<a href="mailto:tomancak@mpi-cbg.de">tomancak@mpi-cbg.de</a>><br></span></div><br><div lang="EN-US" link="#0563C1" vlink="#954F72" style="font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div class="WordSection1" style="page: WordSection1; "><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Dear Dr. Paval,<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">I am Kumar, Graduate student from NTU-Singapore. I referred to your recent article on open SPIM (<a href="http://www.nature.com/nmeth/journal/v10/n7/full/nmeth.2507.html#videos" style="color: rgb(149, 79, 114); text-decoration: underline; ">http://www.nature.com/nmeth/journal/v10/n7/full/nmeth.2507.html#videos</a>) and I find this technique is amazing and I would like to try this method to study developmental biology of bacterial biofilms with reporter strains. I am currently trying this with the custom built SPIM system however I am quite unclear with the carriers for sample fixation, I would like to seek your advice on this.<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">It would be really helpful if you could advise me on the sample preparation techniques for SPIM, if you are using any FEP tubing�s to immobilize the sample kindly advise me on the specifications(i,e tubing material, dimensions and refractive index) of the tubing�s.<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Looking forward to your reply,<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Thank you and have a great day.<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Best regards<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Kumar<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><span lang="EN-SG"> </span></div><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" align="left" width="759" style="width: 569.2pt; margin-left: 6.75pt; margin-right: 6.75pt; "><tbody><tr style="height: 44.25pt; "><td width="150" style="width: 112.75pt; padding: 0in; height: 44.25pt; "><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><span style="color: rgb(31, 73, 125); "><span><image001.gif></span><o:p></o:p></span></div></td><td style="padding: 0in; height: 44.25pt; "><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><span style="font-size: 10pt; color: rgb(31, 73, 125); ">Kumaravel Kandaswamy | Research Student| Nanyang Technological University<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><span style="font-size: 10pt; color: rgb(31, 73, 125); ">Singapore Centre on Environmental Life Sciences Engineering (SCELSE)<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><span style="font-size: 10pt; color: rgb(31, 73, 125); ">SBS-B2N-27, 60 Nanyang Drive, Singapore 637551<br>Tel: (65) 82649175 | Fax: (65) 65156751 | Email:<span class="Apple-converted-space"> </span></span><a href="mailto:kumarave001@e.ntu.edu.sg" style="color: rgb(149, 79, 114); text-decoration: underline; "><span style="font-size: 10pt; ">kumarave001@e.ntu.edu.sg</span></a><span style="font-size: 10pt; color: rgb(31, 73, 125); "><span class="Apple-converted-space"> </span>|Web:<span class="Apple-converted-space"> </span></span><a href="http://www.scelse.sg/" style="color: rgb(149, 79, 114); text-decoration: underline; "><span style="font-size: 10pt; ">www.scelse.sg</span></a><span style="font-size: 10pt; color: rgb(31, 73, 125); "><o:p></o:p></span></div></td></tr></tbody></table><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p></o:p></div></div></div></blockquote></div><br></div></div></div>_______________________________________________<br>OpenSPIM mailing list<br><a href="mailto:OpenSPIM@openspim.org">OpenSPIM@openspim.org</a><br>http://openspim.org/mailman/listinfo/openspim<br></blockquote></div><br></div></body></html>