<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Dear Kumar,<div><br></div><div>we have a paper on zebrafish mounting for SPIM with FEP tubes:</div><div><a href="http://www.ncbi.nlm.nih.gov/pubmed/22872089">http://www.ncbi.nlm.nih.gov/pubmed/22872089</a></div><div><br></div><div>Best</div><div>Jan</div><div> <br><div apple-content-edited="true">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div style="font-size: 11px; ">Dr. Jan Huisken</div><div style="font-size: 11px; "><b>MPI of Molecular Cell Biology and Genetics</b></div><div style="font-size: 11px; ">Pfotenhauerstr. 108, 01307 Dresden, Germany</div></div></span></div>
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<br><div><div>On Oct 6, 2013, at 10:40 AM, Pavel Tomancak wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><meta http-equiv="Content-Type" content="text/html charset=windows-1252"><base href="x-msg://535/"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Forwarding… Do we have a wiki page on the FEP tubes?<div><br></div><div>PAvel<br><div><div><br class="Apple-interchange-newline"><blockquote type="cite"><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;"><span style="font-family:'Helvetica'; font-size:medium; color:rgba(0, 0, 0, 1.0);"><b>From: </b></span><span style="font-family:'Helvetica'; font-size:medium;">#KUMARAVEL KANDASWAMY# <<a href="mailto:KUMARAVE001@e.ntu.edu.sg">KUMARAVE001@e.ntu.edu.sg</a>><br></span></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;"><span style="font-family:'Helvetica'; font-size:medium; color:rgba(0, 0, 0, 1.0);"><b>Subject: </b></span><span style="font-family:'Helvetica'; font-size:medium;"><b>Request for information on open SPIM</b><br></span></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;"><span style="font-family:'Helvetica'; font-size:medium; color:rgba(0, 0, 0, 1.0);"><b>Date: </b></span><span style="font-family:'Helvetica'; font-size:medium;">October 5, 2013 8:33:19 PM GMT+02:00<br></span></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;"><span style="font-family:'Helvetica'; font-size:medium; color:rgba(0, 0, 0, 1.0);"><b>To: </b></span><span style="font-family:'Helvetica'; font-size:medium;">"<a href="mailto:tomancak@mpi-cbg.de">tomancak@mpi-cbg.de</a>" <<a href="mailto:tomancak@mpi-cbg.de">tomancak@mpi-cbg.de</a>><br></span></div><br><div lang="EN-US" link="#0563C1" vlink="#954F72" style="font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div class="WordSection1" style="page: WordSection1; "><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Dear Dr. Paval,<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">I am Kumar, Graduate student from NTU-Singapore. I referred to your recent article on open SPIM (<a href="http://www.nature.com/nmeth/journal/v10/n7/full/nmeth.2507.html#videos" style="color: rgb(149, 79, 114); text-decoration: underline; ">http://www.nature.com/nmeth/journal/v10/n7/full/nmeth.2507.html#videos</a>) and I find this technique is amazing and I would like to try this method to study developmental biology of bacterial biofilms with reporter strains. I am currently trying this with the custom built SPIM system however I am quite unclear with the carriers for sample fixation, I would like to seek your advice on this.<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">It would be really helpful if you could advise me on the sample preparation techniques for SPIM, if you are using any FEP tubing’s to immobilize the sample kindly advise me on the specifications(i,e tubing material, dimensions and refractive index) of the tubing’s.<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Looking forward to your reply,<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Thank you and have a great day.<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Best regards<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; ">Kumar<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><span lang="EN-SG"> </span></div><table class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" align="left" width="759" style="width: 569.2pt; margin-left: 6.75pt; margin-right: 6.75pt; "><tbody><tr style="height: 44.25pt; "><td width="150" style="width: 112.75pt; padding: 0in; height: 44.25pt; "><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><span style="color: rgb(31, 73, 125); "><span><image001.gif></span><o:p></o:p></span></div></td><td style="padding: 0in; height: 44.25pt; "><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><span style="font-size: 10pt; color: rgb(31, 73, 125); ">Kumaravel Kandaswamy | Research Student| Nanyang Technological University<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><span style="font-size: 10pt; color: rgb(31, 73, 125); ">Singapore Centre on Environmental Life Sciences Engineering (SCELSE)<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><span style="font-size: 10pt; color: rgb(31, 73, 125); ">SBS-B2N-27, 60 Nanyang Drive, Singapore 637551<br>Tel: (65) 82649175 | Fax: (65) 65156751 | Email:<span class="Apple-converted-space"> </span></span><a href="mailto:kumarave001@e.ntu.edu.sg" style="color: rgb(149, 79, 114); text-decoration: underline; "><span style="font-size: 10pt; ">kumarave001@e.ntu.edu.sg</span></a><span style="font-size: 10pt; color: rgb(31, 73, 125); "><span class="Apple-converted-space"> </span>|Web:<span class="Apple-converted-space"> </span></span><a href="http://www.scelse.sg/" style="color: rgb(149, 79, 114); text-decoration: underline; "><span style="font-size: 10pt; ">www.scelse.sg</span></a><span style="font-size: 10pt; color: rgb(31, 73, 125); "><o:p></o:p></span></div></td></tr></tbody></table><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif; "><o:p></o:p></div></div></div></blockquote></div><br></div></div></div>_______________________________________________<br>OpenSPIM mailing list<br><a href="mailto:OpenSPIM@openspim.org">OpenSPIM@openspim.org</a><br>http://openspim.org/mailman/listinfo/openspim<br></blockquote></div><br></div></body></html>