[OpenSPIM] [openspim] laser questions

[Wade Sigurdson]

Hi Johannes,

We are making good progress on our Open SPIM project.  Machining almost 
done and we just received the USB Pickard stage.

I'm now trying the get the software working.  I use Fiji all the time 
for image processing (great package and a pleasure to use) and so am 
familiar with installation and operation.  But when I installed the 
OpenSPIM version and opened MicroManager Studio I get the following 
error window:

Failed to open libMMCoreJ_wrap.jnilib
mmcorej.MMCoreJJNI.swig_module_init()V

The MM window opens, but when I try to run the Hardware config wizard I 
get the following:

org.micromanager.MMStudioMainFrame.run HardwareWizard 
(MMStudioMainFrame.java:4763)

which I'm guessing is due to the first error.

I looked around at the MM forum for some ideas on what might be wrong 
and even tried copying the MMCoreJ_wrap.dll into the OpenSPIM root 
directory, but still have the error.

Is there something wrong with my XP environment variables or is there a 
path I need to configure?

Thanks for any help.

Wade
On 03/14/2013 11:58 AM, Johannes Schindelin wrote:
> Hi Wade,
>
> On Thu, 14 Mar 2013, Wade Sigurdson wrote:
>
>> We are planning on building the OpenSPIM system as described on your
>> wiki page and are trying to re-purpose an Ar/Kr laser from an old
>> Bio-Rad Confocal as the illumination source.  For the 488nm line, I
>> measured about 2mW  output from the fiber optic after aligning the laser
>> launch.
>>
>> We could easily design and make a mount for the fiber optic which would
>> replace the Coherent laser in your original plans, but is this enough
>> power to be usable?
> IIRC we often drive it with about 1mW or even less, so you should be fine.
> Note, however, that we needed computer control over the laser in long-term
> time-lapse recordings because the specimen bleached too much otherwise.
>
>> Since this is a proof of principle project and if we get the system
>> working without purchasing the diode laser, we likely could interest
>> other potential uses to contribute money to the project.
>>
>> We also have in hand a Zeiss 63x/1.0 water immersion objective which we
>> may interested in using as a replacement for the Olympus 20x lens. Could
>> this be used with the Olympus tube lens without introducing significant
>> aberrations?  I believe that Zeiss does some chromatic aberration
>> correction in the tube lens of their microscopes, but have no idea if
>> the  Olympus tube lens would be compatible with Zeiss objectives.
> The tube lens is on the illumination side, for which I would stay with a
> 10x lens. On the detection side, we have a 20x objective that might be
> easily replaced by your 63x objective. I am no optics expert and depending
> who I asked, I got encouraging and discouraging answers whether it would
> work to replace the detection objective with a different magnification
> one.
>
> Ciao,
> Johannes
>
>

-- 
*Wade J. Sigurdson, Ph.D.
Director, Confocal Microscope and Flow Cytometry Facility
School of Medicine and Biomedical Sciences
University at Buffalo
*
Voice - 716-829-3589
FAX - 716-829-3589
wjs at buffalo.edu
www.smbs.buffalo.edu/confocal
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