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Hi Johannes,<br>
<br>
We are making good progress on our Open SPIM project. Machining
almost done and we just received the USB Pickard stage.<br>
<br>
I'm now trying the get the software working. I use Fiji all the
time for image processing (great package and a pleasure to use) and
so am familiar with installation and operation. But when I
installed the OpenSPIM version and opened MicroManager Studio I get
the following error window:<br>
<br>
Failed to open libMMCoreJ_wrap.jnilib<br>
mmcorej.MMCoreJJNI.swig_module_init()V<br>
<br>
The MM window opens, but when I try to run the Hardware config
wizard I get the following:<br>
<br>
org.micromanager.MMStudioMainFrame.run HardwareWizard
(MMStudioMainFrame.java:4763)<br>
<br>
which I'm guessing is due to the first error.<br>
<br>
I looked around at the MM forum for some ideas on what might be
wrong and even tried copying the MMCoreJ_wrap.dll into the OpenSPIM
root directory, but still have the error.<br>
<br>
Is there something wrong with my XP environment variables or is
there a path I need to configure?<br>
<br>
Thanks for any help.<br>
<br>
Wade<br>
<div class="moz-cite-prefix">On 03/14/2013 11:58 AM, Johannes
Schindelin wrote:<br>
</div>
<blockquote
cite="mid:alpine.DEB.1.00.1303141653310.3794@s15462909.onlinehome-server.info"
type="cite">
<pre wrap="">Hi Wade,
On Thu, 14 Mar 2013, Wade Sigurdson wrote:
</pre>
<blockquote type="cite">
<pre wrap="">We are planning on building the OpenSPIM system as described on your
wiki page and are trying to re-purpose an Ar/Kr laser from an old
Bio-Rad Confocal as the illumination source. For the 488nm line, I
measured about 2mW output from the fiber optic after aligning the laser
launch.
We could easily design and make a mount for the fiber optic which would
replace the Coherent laser in your original plans, but is this enough
power to be usable?
</pre>
</blockquote>
<pre wrap="">
IIRC we often drive it with about 1mW or even less, so you should be fine.
Note, however, that we needed computer control over the laser in long-term
time-lapse recordings because the specimen bleached too much otherwise.
</pre>
<blockquote type="cite">
<pre wrap="">Since this is a proof of principle project and if we get the system
working without purchasing the diode laser, we likely could interest
other potential uses to contribute money to the project.
We also have in hand a Zeiss 63x/1.0 water immersion objective which we
may interested in using as a replacement for the Olympus 20x lens. Could
this be used with the Olympus tube lens without introducing significant
aberrations? I believe that Zeiss does some chromatic aberration
correction in the tube lens of their microscopes, but have no idea if
the Olympus tube lens would be compatible with Zeiss objectives.
</pre>
</blockquote>
<pre wrap="">
The tube lens is on the illumination side, for which I would stay with a
10x lens. On the detection side, we have a 20x objective that might be
easily replaced by your 63x objective. I am no optics expert and depending
who I asked, I got encouraging and discouraging answers whether it would
work to replace the detection objective with a different magnification
one.
Ciao,
Johannes
</pre>
</blockquote>
<br>
<div class="moz-signature">-- <br>
<b>Wade J. Sigurdson, Ph.D.<br>
Director, Confocal Microscope and Flow Cytometry Facility<br>
School of Medicine and Biomedical Sciences<br>
University at Buffalo<br>
</b><br>
Voice - 716-829-3589<br>
FAX - 716-829-3589<br>
<a class="moz-txt-link-abbreviated" href="mailto:wjs@buffalo.edu">wjs@buffalo.edu</a><br>
<a class="moz-txt-link-abbreviated" href="http://www.smbs.buffalo.edu/confocal">www.smbs.buffalo.edu/confocal</a>
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