[OpenSPIM] Acquisition laser power/camera exposure/et cetera

[Pavel Tomancak]

Hi Luke,

Do you mean the beads are too weak? Typically the beads are the brightest part of the image, unless you use ones that are incompatible with your illumination/emission spectrum. We use the OpenSPIM typically on very low laser power 2-4% and the exposure times are on the order of 50-100ms when imaging strong fluorescent signal. under these conditions the green beads are extremely bright in the green channel.

Can you send me some images? I may be able to help with the registration.

All the best

PAvel
 
On Jul 5, 2013, at 9:05 PM, Luke Stuyvenberg <stuyvenberg at wisc.edu> wrote:

> Hey Pete,
> 
> When you get a moment, could you write back with the typical settings you use for the laser power and camera exposure when acquiring? We acquired some data recently which I'm having some trouble registering; I think the signal is too weak, and I wanted to get an idea of what you guys have been using. Also anything else you think might be relevant to getting a good image, or tips for registering data, would be much appreciated!
> 
> 
> Thanks,
> Luke
> 
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